LOCUS       sequence-151163-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent AAV-mediated expression of GFP under the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 524)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-FLEX-GFP
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(148..181)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(216..235)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(297..319)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtaaa actcgagggg ggatccctag tataacttcg
       61 tatagcatac attatacgaa gttatgggtc gatggtgatg cttggcaatt cgggtcgatg
      121 gtgaagcatt ggtcttccta ttttgatata acttcgtata ggatacttta tacgaagtta
      181 tggggatcca attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg
      241 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc
      301 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc
      361 ggacagcccc gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc
      421 acgcagccac agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg
      481 agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg gtca
//
