LOCUS       sequence-151178-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation Self-inactivating lentivector expressing NS2B from 
            Zika virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  TITLE     Arumugaswami Lab Plasmids
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV_Zika_NS2B_Flag
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             348..528
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    575..700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1193..1426
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1611..1655
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1953..2070
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2148..2359
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2372..2640
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3081..3104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3153..3725
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3320..3337)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3547..3566
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3728..4330
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4227..4247
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4346..4934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4399..4419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4817..4828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5461..5641
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5667..5684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5673..5897
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5943..6371
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6030..6049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6240..6261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6380..6400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6385..6714
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6565..6700
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6627..6646
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6762..6809
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6828..7202
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7332..7453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7369..7388)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7423..7442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7502..7518)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7502..7518)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7515..7537)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7526..7542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7550..7556,7557..7574,7575..7580))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7595..7616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7733..7750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7904..8492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7984..8003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8663..9454,9455..9523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9286..9305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9524..9628)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9703..9722)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9894..10143,1..130)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     2641 caagctgtga ccggcgccta ccaccggtcg ccaccatgag ctggccccct agcgaagtac
     2701 tcacagctgt tggcctgata tgcgcattgg ctggagggtt cgccaaggca gatatagaga
     2761 tggctgggcc catggccgcg gtcggtctgc taattgtcag ttacgtggtc tcaggaaaga
     2821 gtgtggacat gtacattgaa agagcaggtg acatcacatg ggaaaaagat gcggaagtca
     2881 ctggaaacag tccccggctc gatgtggcgc tagatgagag tggtgatttc tccctggtgg
     2941 aggatgacgg tccccccatg agagagatca tactcaaggt ggtcctgatg accatctgtg
     3001 gcatgaaccc aatagccata ccctttgcag ctggagcgtg gtacgtatac gtgaagactg
     3061 gaaaaaggtc cgggatggcc gactacaaag acgacgatga caagtagcac tcgaggatat
     3121 cgacgcgtga agcttagcgg ccgcaaattc cgcccctctc cctccccccc ccctaacgtt
     3181 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     3241 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     3301 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     3361 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     3421 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     3481 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     3541 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     3601 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     3661 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3721 taataccatg gccaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3781 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3841 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3901 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     3961 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggctcgc gcatggccga
     4021 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4081 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4141 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ctggggtgcc
     4201 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4261 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4321 cggtgcctga gttcgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     4381 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4441 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4501 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4561 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4621 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4681 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4741 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4801 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4861 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4921 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     4981 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5041 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     5101 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5161 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     5221 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     5281 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     5341 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     5401 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     5461 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5521 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5581 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5641 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5701 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5761 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5821 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5881 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5941 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6001 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6061 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6121 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6181 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6241 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6301 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6361 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6421 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6541 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6601 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     6661 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6721 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     6781 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     6841 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     6901 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     6961 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     7021 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     7081 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     7141 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     7201 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7261 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7321 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7381 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7441 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7501 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7561 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7621 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7681 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7741 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7801 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7861 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7921 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7981 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8041 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8101 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8161 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8221 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8281 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8341 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8401 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8461 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8521 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8581 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8641 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8701 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8761 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8821 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8881 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8941 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9001 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9061 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9121 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9181 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9241 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9301 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9361 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9421 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9481 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9541 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9601 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9661 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     9721 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9781 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9841 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     9901 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     9961 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    10021 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    10081 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10141 tca
//
