LOCUS       sequence-151199-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. cerevisiae FDC1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   McKenna R, Nielsen DR
  TITLE     Styrene biosynthesis from glucose by engineered E. coli.
  JOURNAL   Metab Eng. 2011 Sep;13(5):544-54. doi: 10.1016/j.ymben.2011.06.005. 
            Epub 2011 Jun 23.
  PUBMED    21722749
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymben.2011.06.005"; journalName: "Metab Eng"; date: 
            "2011-09"; volume: "13"; issue: "5"; pages: "544-54"
COMMENT     Sequence Label: pTfdc1Sc
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(157..162,163..179,180..186)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     199..221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
       61 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
      121 tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tccggctcgt
      181 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagacc atggaattcg
      241 ggaggaatta tatgaggaag ctaaatccag ctttagaatt tagagacttt atccaggtct
      301 taaaagatga agatgactta atcgaaatta ccgaagagat tgatccaaat ctcgaagtag
      361 gtgcaattat gaggaaggcc tatgaatccc acttaccagc cccgttattt aaaaatctca
      421 aaggtgcttc gaaggatctt ttcagcattt taggttgccc agccggttta agaagtaagg
      481 agaaaggaga tcatggtaga attgcccatc atctggggct cgacccaaaa acaactatca
      541 aggaaatcat agattatttg ctggagtgta aggagaagga acctctcccc ccaatcactg
      601 ttcctgtgtc atctgcacct tgtaaaacac atatactttc tgaagaaaaa atacatctac
      661 aaagcctgcc aacaccatat ctacatgttt cagacggtgg caagtactta caaacgtacg
      721 gaatgtggat tcttcaaact ccagataaaa aatggactaa ttggtcaatt gctagaggta
      781 tggttgtaga tgacaagcat atcactggtc tggtaattaa accacaacat attagacaaa
      841 ttgctgactc ttgggcagca attggaaaag caaatgaaat tcctttcgcg ttatgttttg
      901 gcgttccccc agcagctatt ttagttagtt ccatgccaat tcctgaaggt gtttctgaat
      961 cggattatgt tggcgcaatc ttgggtgagt cggttccagt ag
//
