LOCUS       sequence-151200-        5685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S. cerevisiae FDC1 in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   McKenna R, Nielsen DR
  TITLE     Styrene biosynthesis from glucose by engineered E. coli.
  JOURNAL   Metab Eng. 2011 Sep;13(5):544-54. doi: 10.1016/j.ymben.2011.06.005. 
            Epub 2011 Jun 23.
  PUBMED    21722749
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymben.2011.06.005"; journalName: "Metab Eng"; date: 
            "2011-09"; volume: "13"; issue: "5"; pages: "544-54"
COMMENT     Sequence Label: pTfdc1Sc
FEATURES             Location/Qualifiers
     source          1..5685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(193..198,199..215,216..222)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     235..257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(1868..1885)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2038..2124
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2216..2243
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2263..2354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2355..2423,2424..3215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2573..2592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3386..3974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3875..3894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4128..4145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4160..4300
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4226..4245)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4386..4408
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        4486..4563
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4564..5646
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4583..4602)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg aattcgggag gaattatatg aggaagctaa
      301 atccagcttt agaatttaga gactttatcc aggtcttaaa agatgaagat gacttaatcg
      361 aaattaccga agagattgat ccaaatctcg aagtaggtgc aattatgagg aaggcctatg
      421 aatcccactt accagccccg ttatttaaaa atctcaaagg tgcttcgaag gatcttttca
      481 gcattttagg ttgcccagcc ggtttaagaa gtaaggagaa aggagatcat ggtagaattg
      541 cccatcatct ggggctcgac ccaaaaacaa ctatcaagga aatcatagat tatttgctgg
      601 agtgtaagga gaaggaacct ctccccccaa tcactgttcc tgtgtcatct gcaccttgta
      661 aaacacatat actttctgaa gaaaaaatac atctacaaag cctgccaaca ccatatctac
      721 atgtttcaga cggtggcaag tacttacaaa cgtacggaat gtggattctt caaactccag
      781 ataaaaaatg gactaattgg tcaattgcta gaggtatggt tgtagatgac aagcatatca
      841 ctggtctggt aattaaacca caacatatta gacaaattgc tgactcttgg gcagcaattg
      901 gaaaagcaaa tgaaattcct ttcgcgttat gttttggcgt tcccccagca gctattttag
      961 ttagttccat gccaattcct gaaggtgttt ctgaatcgga ttatgttggc gcaatcttgg
     1021 gtgagtcggt tccagtagta aaatgtgaga ccaacgattt aatggttcct gcaacgagtg
     1081 agatggtatt tgagggtact ttgtccttaa cagatacaca tctggaaggc ccatttggtg
     1141 agatgcatgg atatgttttc aaaagccaag gtcatccttg tccattgtac actgtcaagg
     1201 ctatgagtta cagagacaat gctattctac ctgtttcgaa ccccggtctt tgtacggatg
     1261 agacacatac cttgattggt tcactagtgg ctactgaggc caaggagctg gctattgaat
     1321 ctggcttgcc aattctggat gcctttatgc cttatgaggc tcaggctctt tggcttatct
     1381 taaaggtgga tttgaaaggg ctgcaagcat tgaagacaac gcctgaagaa ttttgtaaga
     1441 aggtaggtga tatttacttt aggacaaaag ttggttttat agtccatgaa ataattttgg
     1501 tggcagatga tatcgacata tttaacttca aagaagtcat ctgggcctac gttacaagac
     1561 atacacctgt tgcagatcag atggcttttg atgatgtcac ttcttttcct ttggctccct
     1621 ttgtttcgca gtcatccaga agtaagacta tgaaaggtgg aaagtgcgtt actaactgca
     1681 tatttagaca gcaatatgag cgcagttttg actacataac ttgtaatttt gaaaagggat
     1741 atccaaaagg attagttgac aaagtaaatg aaaattggaa aaggtacgga tataaataag
     1801 gatcctctag agtcgacctg caggcatgca agcttggctg ttttggcgga tgagagaaga
     1861 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc
     1921 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc
     1981 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa
     2041 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2101 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     2161 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag
     2221 gccatcctga cggatggcct ttttgcgttt ctacaaactc tttttgttta tttttctaaa
     2281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2581 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2881 gtgacaccac gatgcctaca gcaatggcaa caacgttgcg caaactatta actggcgaac
     2941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4141 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4201 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4261 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     4321 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4381 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     4441 gcagatcaat tcgcgcgcga aggcgaagcg gcatgcattt acgttgacac catcgaatgg
     4501 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
     4561 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     4621 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     4681 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     4741 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     4801 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     4861 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     4921 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     4981 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     5041 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     5101 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     5161 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     5221 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     5281 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     5341 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     5401 tcatgttata tcccgccgtc aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     5461 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     5521 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     5581 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5641 cagtgagcgc aacgcaatta atgtgagtta gcgcgaattg atctg
//
