LOCUS       sequence-151214-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli Mechanosensitive Channel of Small Conductance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Bass RB, Strop P, Barclay M, Rees DC
  TITLE     Crystal structure of Escherichia coli MscS, a voltage-modulated and 
            mechanosensitive channel.
  JOURNAL   Science. 2002 Nov 22;298(5598):1582-7.
  PUBMED    12446901
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2002-11-22"; volume: "298(5598)"; pages: "1582-7"
COMMENT     Sequence Label: EcMscS
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(49..96)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     92..110
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(163..180)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tacagggcgc gtcccattcg ccaatccgga tatagttcct cctttcagca aaaaacccct
       61 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
      121 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      181 ctcgagtgcg gccgcaagct tgtcgacgga gctcgaattc ggatccagag agtattatct
      241 ggcctcagcg ttgattacgc agctttgtct tctttcaccc gcttaaagtt cacatccatt
      301 tgcgggtacg ggaagctgat accggcggca tcaaattcac gtttaatacg ctccagcaca
      361 tcccagtaca cgttttgcag atcgccgctg ttgctccaga cgcggaccac gaaattaatc
      421 gacgatgcac caagttcgtt caggcgcaca gtcatttcgc gatctttcaa aatgcgatct
      481 tcagactgga taatattggt caggatctgc ttaacctgat cgatatcgga atcatacgcc
      541 acgccaataa taaattcgtt acggcgaact ggctcgcggg agaagttaat aatatttccg
      601 gcaataattt taccgttcgg aataacgata attttaccgt ctgcagtacg catggtggtg
      661 gagaaaatct gcacactcag cacagtaccg gctacgccgc ccaggtcaac atattctccg
      721 gcacggaacg ggcggaacat gacaagtaac acgccagcgg ccaggttaga cagtgacccc
      781 tgcaaagcca gaccaacagc taagcctgcg gcaccgagta cagcaatgac tgacgcggtt
      841 tgtacaccc
//
