LOCUS       sequence-151218-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable expression of shRNA targeting mouse Pnky. The shRNA (and 
            EGFP) can be excised by the addition of Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Ramos AD, Andersen RE, Liu SJ, Nowakowski TJ, Hong SJ, Gertz CC, 
            Salinas RD, Zarabi H, Kriegstein AR, Lim DA
  TITLE     The long noncoding RNA Pnky regulates neuronal differentiation of 
            embryonic and postnatal neural stem cells.
  JOURNAL   Cell Stem Cell. 2015 Apr 2;16(4):439-47. doi: 
            10.1016/j.stem.2015.02.007. Epub 2015 Mar 19.
  PUBMED    25800779
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.02.007"; journalName: "Cell Stem Cell"; date: 
            "2015-04-2- 2"; volume: "16"; issue: "4"; pages: "439-47"
COMMENT     Sequence Label: pSicoR (EGFP) shPnky-2
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(297..330)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     334..354
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     enhancer        586..889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
       61 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gagatccgac gcgccatctc
      121 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc
      181 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac
      241 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa
      301 cttcgtatag catacattat acgaagttat aaacagcaca aaaggaaact caccctaact
      361 gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgttgatga
      421 cgtggagagg atttttcaag agaaaatcct ctccacgtca tcttttttct cgagtactag
      481 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcattagtt attaatagta
      541 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      601 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      661 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      721 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      781 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      841 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      901 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      961 ccccattgac gtcaatggga gtttgttt
//
