LOCUS       sequence-151219-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses  Coumarinyl- tRNA synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Steinberg X, Galpin J, Nasir G, Sepulveda-Ugarte J, Sepulveda R, 
            Gonzalez-Nilo F, Islas LD, Ahern CA, Brauchi SE
  TITLE     A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically 
            Encoded Fluorescent Amino Acids
  JOURNAL   bioRxiv, 10.1101/065227
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            10.1101/065227"
COMMENT     Sequence Label: pCDNA3.1_CoumRS
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 ccaccatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg gtagcccagg
      181 tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg ctcggttgcg
      241 gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg gaatgcctga
      301 aacgcttcca gcaggcgggc cacaagccgg ttgcgcacgt aggcggcgcg gggggtctga
      361 ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa actgttcagg
      421 agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac tgtggagaaa
      481 actatcatat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg ctgaccttcc
      541 tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa gcggttaagc
      601 agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac aacctgttgc
      661 agggttatgg catcgcctgt gggaacaaac agtacggtgt ggtgctgcaa attggtggtt
      721 ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg catcagaatc
      781 aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga tggcaccaaa tttggtaaaa
      841 ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa ttctaccagt
      901 tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc acctttatga
      961 gcattgaaga gatcaacgcc ctggaagaag aagataaaa
//
