LOCUS       sequence-151220-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses  Coumarinyl- tRNA synthetase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Steinberg X, Galpin J, Nasir G, Sepulveda-Ugarte J, Sepulveda R, 
            Gonzalez-Nilo F, Islas LD, Ahern CA, Brauchi SE
  TITLE     A Rationally Designed Aminoacyl-tRNA Synthetase for Genetically 
            Encoded Fluorescent Amino Acids
  JOURNAL   bioRxiv, 10.1101/065227
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            10.1101/065227"
COMMENT     Sequence Label: pCDNA3.1_CoumRS
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    113..337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     326..343
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(378..401)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      121 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      181 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      241 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      301 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
      361 acgggccctc tagattactt gtcgtcatcg tctttgtagt ctttccagca aatcagacag
      421 taattctttt taccgcgacg cagtaaggta aaacgaccaa acagacgatc ttcttcttta
      481 aagaagtatt caggatcgga ctgtttttca ccgttaatgg tgatggcatt ggaggcgata
      541 gttttacgtg cctgaccacg ggaaggttgc agttcagaat cgaccagtgc ctgcatcagg
      601 tctgcgccct tttccatctc aaccatcggt acgccgtcct gcgccagctg ttcgaagtcc
      661 gcttcactca gcgcactcaa agaaccgctg aacaggcatt cggtaatacg ttttgccgcc
      721 tgtaaacctt cttcaccgtg aaccagacga gtcacctgct ccgccagtac atactgggcg
      781 cgcggtgctt taccgctgtt tttatcttct tcttccaggg cgttgatctc ttcaatgctc
      841 ataaaggtga agaacttcag gaagcggtaa acgtcggcat ccgcagtgtt gatccagaac
      901 tggtagaatt tgtacgggct ggttttcttc ggatccaacc agactgcgcc gccttcagtt
      961 ttaccaaatt tggtgccatc tgctt
//
