LOCUS       sequence-151228-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of TCOF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Shiang lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmTCOF1
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(664..680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(664..680)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(717..737)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(717..735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(756..772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(769..791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    780..796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(804..810,811..829,830..835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 actgctgcct tggcccgctg acaccgttcc agacttggca gtgggtccga gggcttgggc
       61 attgccctcc tcctcagttt ctgaggccaa gacagtgttt gccaagggct ctgctgactt
      121 tttgggtgac ttcccagaga gcaggtggac caccgtcttt ccggacccag ggtgcgacac
      181 agagttcacc gtcttgttgg ctgtcttggt ctttcccttt tcttttactt ttgatggcaa
      241 agctgcggtt gcagaattga caggtgtcgg tcttggggtg gctttggccc tttcggttgc
      301 tgcctcctct tccttctcct gatccgagct ctctgagctg ctaacaggat ccgacactcg
      361 agacttctta gcctgaaggg tctcatcatc ctctgccttc tgcttctggc caagctctga
      421 ggtctgttgc cagtgtgtat agatgtccag aagggtgacg ggctgagtca ggaaactctt
      481 ctggccgctc tgctccttta cttcccgcgc cgcgcggacg tagcctgctt gcaacagatg
      541 atggtaaatg aggggaagca gctcccgccg cttcctggcc tcggccatgc cagagatctg
      601 gccaggggat tcacgtgcac gccccctcgc ccccgcctcg tgccgaattc ctgcagcccg
      661 ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
      721 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      781 tgttatccgc tcacaattcc acacaacata cgagccggaa agcataaagt gtaaagcctg
      841 gg
//
