LOCUS       sequence-151232-         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an AHL-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pLux01
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             174..185
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    231..252
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(254..276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      291..377
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atatcagatt taaccataga atgaggataa atgatcgcga gtaaataata ttcacaatgt
       61 accattttag tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta
      121 attttattaa ttattctgta tgtgtcgtcg gcatttatgt ttttcatcta gtatttctcc
      181 tctttctcta gtaggcgcta tcatgccata ccgcgaaagg ttttgcacca ctctagaagc
      241 ggccgcgaat tctcctgtgt gaaattgtta tccgctttaa ttaaaggcat caaataaaac
      301 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      361 tcctgagtag gacaaatccg ccgccctaga cagctgggcg cgccccccct acgggcttgc
      421 tctccgggct tcgccctgcg cggtcgctgc gctcccttgc cagcccgtgg atatgtggac
      481 gatggccgcg agcggccacc ggctggctcg cttc
//
