LOCUS       sequence-151233-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330A-1x2
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(35..283)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(93..112)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(263..283)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(425..444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttctagctc taaaacaggt cttctcgaag acccggtgtt tcgtcctttc cacaagatat
       61 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
      121 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
      181 gtactaatat ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt
      241 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcacatgtg agcaaaaggc
      301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      781 agaag
//
