LOCUS       sequence-151239-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one CRISPR/Cas9 vector with CAG promoter for expression in 
            human ESC/iPSC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Stoehr A, Kennedy L, Yang Y, Patel S, Lin Y, Linask KL, Fergusson M,
            Zhu J, Gucek M, Zou J, Murphy E
  TITLE     The ribosomal prolyl-hydroxylase OGFOD1 decreases during cardiac 
            differentiation and modulates translation and splicing.
  JOURNAL   JCI Insight. 2019 May 21;5. pii: 128496. doi: 
            10.1172/jci.insight.128496.
  PUBMED    31112528
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2019-05-21"; volume: "5. pii: 128496. doi"; pages:
            " 10.1172/jci.insight.128496"
COMMENT     Sequence Label: pCAG-SpCas9-GFP-U6-gRNA
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggattcttct cgaagctgct tctttccatg atggtgatcc ccagcagctc tttcacactc
       61 ttcagtttct tggacttgcc cttttccact ttggccacca ccagcacaga ataggccacg
      121 gtggggctgt cgaagccgcc gtacttctta gggtcccagt ccttctttct ggcgatcagc
      181 ttatcgctgt tcctcttggg caggatagac tctttgctga agccgcctgt ctgcacctcg
      241 gtctttttca cgatattcac ttggggcatg ctcagcactt tccgcacggt ggcaaaatcc
      301 cggcccttat cccacacgat ctccccggtt tcgccgtttg tctcgatcag aggccgcttc
      361 cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa agttcatgat gttgctgtag
      421 aagaagtact tggcggtagc cttgccgatt tcctgctcgc tcttggcgat catcttccgc
      481 acgtcgtaca ccttgtagtc gccgtacacg aactcgcttt ccagcttagg gtactttttg
      541 atcagggcgg ttcccacgac ggcgttcagg taggcgtcgt gggcgtggtg gtagttgttg
      601 atctcgcgca ctttgtaaaa ctggaaatcc ttccggaaat cggacaccag cttggacttc
      661 agggtgatca ctttcacttc ccggatcagc ttgtcattct cgtcgtactt agtgttcatc
      721 cgggagtcca ggatctgtgc cacgtgcttt gtgatctgcc gggtttccac cagctgtctc
      781 ttgatgaagc cggccttatc cagttcgctc aggccgcctc tctcggcctt ggtcagattg
      841 tcgaactttc tctgggtaat cagcttggcg ttcagcagct gccgccagta gttcttcatc
      901 ttcttcacga cctcttcgga gggcacgttg tcgctcttgc cccggttctt g
//
