LOCUS       sequence-151241-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plasmid that constitutively expresses human myogenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     pWPI-myogenin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWPI-myogenin
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(134..136,137..139,140..850)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(134..136,137..139,140..850)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(179..200)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(440..459)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     787..808
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(929..945)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(930..946)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
       61 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
      121 cgatgataat accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      181 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      241 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      301 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      361 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      421 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      481 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      541 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      601 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      661 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      721 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      781 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      841 gctgtacaag tccggactca gatctcgact agctagtagc tagctagcta gtcgagctca
      901 agcttcgaat tcgatatcaa gcttatcgcg ataccgtcga cctcgaggga attccgataa
      961 tcaacctctg gattacaaaa tttgtgaaag attgactgg
//
