LOCUS       sequence-151242-       11800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that constitutively expresses human myogenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11800)
  TITLE     pWPI-myogenin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWPI-myogenin
FEATURES             Location/Qualifiers
     source          1..11800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    711..1278
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    729..1280
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(873..890)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1100..1119
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1283..1285,1286..1288,1289..1999)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1283..1285,1286..1288,1289..1999)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1328..1349)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1589..1608)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1936..1957
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2078..2094)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2079..2095)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2108..2696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2161..2181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2579..2590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2865..2898)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2918..3098
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3123..3141)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3124..3141)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3431..3450)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3664..3683)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3783..3805
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3843..3861)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3929..4033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4034..4102,4103..4894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4252..4271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5065..5653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5554..5573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5807..5824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5894..5914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5899..6228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6079..6214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7362..7427
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7557..7577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8002..8136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8039..8058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8093..8112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8305..8938
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8985..9110
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9603..9836
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10021..10065
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10423..11600
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10654..11591
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11548..11568
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11648..11765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgcgccatgg agctgtatga gacatccccc tacttctacc aggaaccccg cttctatgat
       61 ggggaaaact acctgcctgt ccacctccag ggcttcgaac caccaggcta cgagcggacg
      121 gagctcaccc tgagccccga ggccccaggg ccccttgagg acaaggggct ggggaccccc
      181 gagcactgtc caggccagtg cctgccgtgg gcgtgtaagg tgtgtaagag gaagtcggtg
      241 tccgtggacc ggcggcgggc ggccacactg agggagaagc gcaggctcaa gaaggtgaat
      301 gaggccttcg aggccctgaa gagaagcacc ctgctcaacc ccaaccagcg gctgcccaag
      361 gtggagatcc tgcgcagtgc catccagtac atcgagcgcc tccaggccct gctcagctcc
      421 ctcaaccagg aggagcgtga cctccgctac cggggcgggg gcgggcccca gccaggggtg
      481 cccagcgaat gcagctctca cagcgcctcc tgcagtccag agtggggcag tgcactggag
      541 ttcagcgcca acccagggga tcatctgctc acggctgacc ctacagatgc ccacaacctg
      601 cactccctca cctccatcgt ggacagcatc acagtggaag atgtgtctgt ggccttccca
      661 gatgaaacca tgcccaactg acatatgccc aaactacggg ctgcaggaat tccgcccccc
      721 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
      781 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
      841 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
      901 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
      961 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     1021 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     1081 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     1141 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     1201 tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     1261 tgaaaaacac gatgataata ccatggtgag caagggcgag gagctgttca ccggggtggt
     1321 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1381 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1441 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1501 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1561 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1621 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1681 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1741 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1801 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1861 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1921 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1981 catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct agctagctag
     2041 tcgagctcaa gcttcgaatt cgatatcaag cttatcgcga taccgtcgac ctcgagggaa
     2101 ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2161 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2221 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2281 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2341 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2401 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2461 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     2521 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2581 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2641 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     2701 ggaattcgct caagcttcga attaattctg cagagctcgg tacctttaag accaatgact
     2761 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2821 attcactccc aacgaagaca agatgggatc aattcaccat gggaataact tcgtatagca
     2881 tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat
     2941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3061 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     3121 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     3181 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     3241 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     3301 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     3361 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     3421 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     3481 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     3541 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     3601 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     3661 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     3721 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3781 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3841 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3901 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3961 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4021 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4081 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4141 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4201 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4261 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4321 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4381 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4441 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4501 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4561 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4621 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4681 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4741 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4801 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4861 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4921 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4981 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     5461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5761 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5821 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5881 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6001 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6061 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6181 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     6241 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     6301 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     6361 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     6421 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     6481 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     6541 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     6601 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     6661 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     6721 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     6781 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     6841 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     6901 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     6961 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     7021 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     7081 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     7141 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     7201 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     7261 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
     7321 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     7381 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     7441 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     7501 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     7561 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     7621 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     7681 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     7741 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     7801 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     7861 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     7921 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     7981 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     8041 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8101 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     8161 aatcatccca aacttcccac cccataccct attaccactg ccaattacct agtggtttca
     8221 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     8281 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     8341 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     8401 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     8461 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     8521 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     8581 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     8641 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     8701 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     8761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8941 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9001 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9061 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9121 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     9181 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     9241 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9301 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9361 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9421 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9481 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9541 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9601 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9661 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9721 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9781 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9841 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9901 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9961 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10021 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10081 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10141 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    10201 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    10261 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
    10321 tatcgatgtc gacgataagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
    10381 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
    10441 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
    10501 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
    10561 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
    10621 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
    10681 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac tggctgcagt
    10741 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
    10801 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
    10861 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
    10921 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
    10981 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
    11041 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
    11101 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
    11161 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
    11221 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
    11281 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
    11341 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
    11401 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
    11461 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    11521 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    11581 ttccatttca ggtgtcgtga ggaatttcga catttaaatt taattaatct cgacggtatc
    11641 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    11701 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    11761 attttatcga tcacgagact agcctcgagg tttgggttgg
//
