LOCUS       sequence-151247-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Experimental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO-mCherry MALAT1_Enhancer.3
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..250
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2..22
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     173..192
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        272..347
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        354..568
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     489..508
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        597..672
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    728..845
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
       61 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      121 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      181 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      241 acgaaacacc ggcaggtcac gcaggccccc agttttagag ctagaaatag caagttaaaa
      301 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgaacgct
      361 gacgtcatca acccgctcca aggaatcgcg ggcccagtgt cactaggcgg gaacacccag
      421 cgcgcgtgcg ccctggcagg aagatggctg tgagggacag gggagtggcg ccctgcaata
      481 tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatgtct ttggatttgg
      541 gagtcttata agttctgtat gagaccactc tttcccagtt ctgcctcagc tcaggagttt
      601 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      661 ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattca
      721 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      781 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      841 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc
      901 gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct
      961 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     1021 ccacagtccc cgagaagttg ggg
//
