LOCUS       sequence-151248-        9310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p4947 pcDNA4c hBrd4 full-length
FEATURES             Location/Qualifiers
     source          1..9310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    917..1079
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             1092..1109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1113..1145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1149..1157,1158..1172)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             3396..3413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5318..5335)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5324..5548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5594..6022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5681..5700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5891..5912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6031..6051)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6036..6365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6216..6351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6278..6297
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6413..6460
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6479..6853
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6983..7104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7020..7039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7074..7093
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7153..7169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7153..7169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7166..7188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7177..7193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7201..7207,7208..7225,7226..7231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7246..7267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7384..7401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7555..8143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7635..7654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8314..9105,9106..9174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8937..8956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9175..9279)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc
      961 cgggcctcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct
     1021 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca
     1081 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa
     1141 tgggtcggga tctgtacgac gatgacgata aggtaccagg atccatgtct gcggagagcg
     1201 gccctgggac gagattgaga aatctgccag taatggggga tggactagaa acttcccaaa
     1261 tgtctacaac acaggcccag gcccaacccc agccagccaa cgcagccagc accaaccccc
     1321 cgcccccaga gacctccaac cctaacaagc ccaagaggca gaccaaccaa ctgcaatacc
     1381 tgctcagagt ggtgctcaag acactatgga aacaccagtt tgcatggcct ttccagcagc
     1441 ctgtggatgc cgtcaagctg aacctccctg attactataa gatcattaaa acgcctatgg
     1501 atatgggaac aataaagaag cgcttggaaa acaactatta ctggaatgct caggaatgta
     1561 tccaggactt caacactatg tttacaaatt gttacatcta caacaagcct ggagatgaca
     1621 tagtcttaat ggcagaagct ctggaaaagc tcttcttgca aaaaataaat gagctaccca
     1681 cagaagaaac cgagatcatg atagtccagg caaaaggaag aggacgtggg aggaaagaaa
     1741 cagggacagc aaaacctggc gtttccacgg taccaaacac aactcaagca tcgactcctc
     1801 cgcagaccca gacccctcag ccgaatcctc ctcctgtgca ggccacgcct caccccttcc
     1861 ctgccgtcac cccggacctc atcgtccaga cccctgtcat gacagtggtg cctccccagc
     1921 cactgcagcc ccccccgcca gtgccccccc agccacaacc cccacccgct ccagctcccc
     1981 agcccgtaca gagccaccca cccatcatcg cggccacccc acagcctgtg aagacaaaga
     2041 agggagtgaa gaggaaagca gacaccacca cccccaccac cattgacccc attcacgagc
     2101 caccctcgct gcccccggag cccaagacca ccaagctggg ccagcggcgg gagagcagcc
     2161 ggcctgtgaa acctccaaag aaggacgtgc ccgactctca gcagcaccca gcaccagaga
     2221 agagcagcaa ggtctcggag cagctcaagt gctgcagcgg catcctcaag gagatgtttg
     2281 ccaagaagca cgccgcctac gcctggccct tctacaagcc tgtggacgtg gaggcactgg
     2341 gcctacacga ctactgtgac atcatcaagc accccatgga catgagcaca atcaagtcta
     2401 aactggaggc ccgtgagtac cgtgatgctc aggagtttgg tgctgacgtc cgattgatgt
     2461 tctccaactg ctataagtac aaccctcctg accatgaggt ggtggccatg gcccgcaagc
     2521 tccaggatgt gttcgaaatg cgctttgcca agatgccgga cgagcctgag gagccagtgg
     2581 tggccgtgtc ctccccggca gtgccccctc ccaccaaggt tgtggccccg ccctcatcca
     2641 gcgacagcag cagcgatagc tcctcggaca gtgacagttc gactgatgac tctgaggagg
     2701 agcgagccca gcggctggct gagctccagg agcagctcaa agccgtgcac gagcagcttg
     2761 cagccctctc tcagccccag cagaacaaac caaagaaaaa ggagaaagac aagaaggaaa
     2821 agaaaaaaga aaagcacaaa aggaaagagg aagtggaaga gaataaaaaa agcaaagcca
     2881 aggaacctcc tcctaaaaag acgaagaaaa ataatagcag caacagcaat gtgagcaaga
     2941 aggagccagc gcccatgaag agcaagcccc ctcccacgta tgagtcggag gaagaggaca
     3001 agtgcaagcc tatgtcctat gaggagaagc ggcagctcag cttggacatc aacaagctcc
     3061 ccggcgagaa gctgggccgc gtggtgcaca tcatccagtc acgggagccc tccctgaaga
     3121 attccaaccc cgacgagatt gaaatcgact ttgagaccct gaagccgtcc acactgcgtg
     3181 agctggagcg ctatgtcacc tcctgtttgc ggaagaaaag gaaacctcaa gctgagaaag
     3241 ttgatgtgat tgccggctcc tccaagatga agggcttctc gtcctcagag tcggagagct
     3301 ccagtgagtc cagctcctct gacagcgaag actccgaaac agagatggct ccgaagtcaa
     3361 aaaagaaggg gcaccccggg agggagcaga agaagcacca tcatcaccac catcagcaga
     3421 tgcagcaggc cccggctcct gtgccccagc agccgccccc gcctccccag cagcccccac
     3481 cgcctccacc tccgcagcag caacagcagc cgccaccccc gcctccccca ccctccatgc
     3541 cgcagcaggc agccccggcg atgaagtcct cgcccccacc cttcattgcc acccaggtgc
     3601 ccgtcctgga gccccagctc ccaggcagcg tctttgaccc catcggccac ttcacccagc
     3661 ccatcctgca cctgccgcag cctgagctgc cccctcacct gccccagccg cctgagcaca
     3721 gcactccacc ccatctcaac cagcacgcag tggtctctcc tccagctttg cacaacgcac
     3781 taccccagca gccatcacgg cccagcaacc gagccgctgc cctgcctccc aagcccgccc
     3841 ggcccccagc cgtgtcacca gccttgaccc aaacacccct gctcccacag ccccccatgg
     3901 cccaaccccc ccaagtgctg ctggaggatg aagagccacc tgccccaccc ctcacctcca
     3961 tgcagatgca gctgtacctg cagcagctgc agaaggtgca gccccctacg ccgctactcc
     4021 cttccgtgaa ggtgcagtcc cagcccccac cccccctgcc gcccccaccc cacccctctg
     4081 tgcagcagca gctgcagcag cagccgccac cacccccacc accccagccc cagcctccac
     4141 cccagcagca gcatcagccc cctccacggc ccgtgcactt gcagcccatg cagttttcca
     4201 cccacatcca acagcccccg ccaccccagg gccagcagcc cccccatccg cccccaggcc
     4261 agcagccacc cccgccgcag cctgccaagc ctcagcaagt catccagcac caccattcac
     4321 cccggcacca caagtcggac ccctactcaa ccggtcacct ccgcgaagcc ccctccccgc
     4381 ttatgataca ttccccccag atgtcacagt tccagagcct gacccaccag tctccacccc
     4441 agcaaaacgt ccagcctaag aaacaggagc tgcgtgctgc ctccgtggtc cagccccagc
     4501 ccctcgtggt ggtgaaggag gagaagatcc actcacccat catccgcagc gagcccttca
     4561 gcccctcgct gcggccggag ccccccaagc acccggagag catcaaggcc cccgtccacc
     4621 tgccccagcg gccggaaatg aagcctgtgg atgtcgggag gcctgtgatc cggcccccag
     4681 agcagaacgc accgccacca ggggcccctg acaaggacaa acagaaacag gagccgaaga
     4741 ctccagttgc gcccaaaaag gacctgaaaa tcaagaacat gggctcctgg gccagcctag
     4801 tgcagaagca tccgaccacc ccctcctcca cagccaagtc atccagcgac agcttcgagc
     4861 agttccgccg cgccgctcgg gagaaagagg agcgtgagaa ggccctgaag gctcaggccg
     4921 agcacgctga gaaggagaag gagcggctgc ggcaggagcg catgaggagc cgagaggacg
     4981 aggatgcgct ggagcaggcc cggcgggccc atgaggaggc acgtcggcgc caggagcagc
     5041 agcagcagca gcgccaggag caacagcagc agcagcaaca gcaagcagct gcggtggctg
     5101 ccgccgccac cccacaggcc cagagctccc agccccagtc catgctggac cagcagaggg
     5161 agttggcccg gaagcgggag caggagcgaa gacgccggga agccatggca gctaccattg
     5221 acatgaattt ccagagtgat ctattgtcaa tatttgaaga aaatcttttc tgagcggccg
     5281 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5341 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5401 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5461 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5521 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5581 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6001 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6061 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6181 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6241 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6301 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6361 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     6421 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     6481 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     6541 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     6601 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     6661 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     6721 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     6781 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     6841 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     6901 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6961 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7021 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7081 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7141 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9301 acctgacgtc
//
