LOCUS       sequence-151256-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-VC3AI
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             4..272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(285..496)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(541..570)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttggatctgt aacggcgcag aacagaaaac gaaacaaaga cgtagagttg agcaagcagg
       61 gtcaggcaaa gcgtggagag ccggctgagt ctaggtaggc tccaagggag cgccggacaa
      121 aggcccggtc tcgacctgag ctttaaactt acctagacgg cggacgcagt tcaggaggca
      181 ccacaggcgg gaggcggcag aacgcgactc aaccggcgtg gatggcggcc tcaggtaggg
      241 cggcgggcgc gtgaaggaga gatgcgagcc cctcgaagct tcagctgtgt tctggcggca
      301 aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca
      361 ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac
      421 cttctctagg cacccgttca attgccgacc cctcccccca acttctcggg gactgtgggc
      481 gatgtgcgct ctgcccactg acgggcaccg gagcgatcgc agatccttcg cggccgctta
      541 caggtcctcc tcgctgatca gcttctgctc gttaggcagg ttgtccaccc tcatcaggtc
      601 cagctccctc tcgaagatct cgtcgatagg cagcatctgg ccgtccacgg tcatgaactt
      661 gtggtccttg gtggccctga tcaggctgcc gtcctccagg cagtactcga acacctcctg
      721 ctcgcccctg tcgtgccact gggccacagg ctgggtgtag atgttgccgt tgttgtccac
      781 gctgtacacg gtgcactcga tcctcttctc cacgatcttg ccgataggca gcaggccgta
      841 ctccacggtc aggatctcgg tctcgtagct caggcagtac tcggcggatc cggcggtgat
      901 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      961 cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac
     1021 ctcggcgcgg gtct
//
