LOCUS       sequence-151258-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-RC3AI
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             2..270
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(283..494)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ggatctgtaa cggcgcagaa cagaaaacga aacaaagacg tagagttgag caagcagggt
       61 caggcaaagc gtggagagcc ggctgagtct aggtaggctc caagggagcg ccggacaaag
      121 gcccggtctc gacctgagct ttaaacttac ctagacggcg gacgcagttc aggaggcacc
      181 acaggcggga ggcggcagaa cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg
      241 gcgggcgcgt gaaggagaga tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa
      301 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
      361 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
      421 tctctaggca cccgttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
      481 tgtgcgctct gcccactgac gggcaccgga gcgatcgcag atccttcgcg gccgcttaca
      541 cattaatact agccgaagca caggctgctc tgtggcgttt catcccactc tcccaggcac
      601 aagacacagg cagggtgctg gcatcctgtt cctctacttc gggtgggaag tcggggttag
      661 gcaggttgtc caccctcatc aggtccagct ccctctcgaa gatctcgtcg ataggcagca
      721 tctggccgtc cacggtcatg aacttgtggt ccttggtggc cctgatcagg ctgccgtcct
      781 ccaggcagta ctcgaacacc tcctgctcgc ccctgtcgtg ccactgggcc acaggctggg
      841 tgtagatgtt gccgttgttg tccacgctgt acacggtgca ctcgatcctc ttctccacga
      901 tcttgccgat aggcagcagg ccgtactcca cggtcaggat ctcggtctcg tagctcaggc
      961 agtactcggc ggatccgtcc tcggggtaca tccgctcgga ggagg
//
