LOCUS       sequence-151262-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctatttcta gctctaaaac agagacgtac aaaaaagagc aagaagctaa aaaagattta
       61 aaaattattt ttagcgcagt taatggaaca ggaactaaat ttaccccaaa aatattacgt
      121 gaatcaggat ataacgttat tgaggttgaa gagcatgcat ttgaagatga aacatttaaa
      181 aatgttgtaa atccaaatcc agaatttgat cctgcatgaa aaataccgct tgaatatggt
      241 attaaacatg atgcagatat tattattatg aatgacccag atgctgacag atttggaatg
      301 gcaataaaac atgatggtca ttttgtaaga ttagatggaa atcaaacagg accaatttta
      361 attgattgaa aattatcaaa tctaaaacgc ttaaatagca ttccaaaaaa tccggctcta
      421 tattcaagtt ttgtaacaag tgatttgggt gatagaatcg ctcatgaaaa atatggagtt
      481 aatattgtaa aaactttaac tggatttaaa tgaatgggta gagaaattgc taaagaagaa
      541 gataacggat taaattttgt ttttgcttat gaagaaagtt atggatatgt aattgatgac
      601 tcagctagag ataaagatgg aatacaagct tctatattaa tagcagaggc tgcttgattt
      661 tataaaaaac aaaataaaac attagtagac tatttagaag atttatttaa agaaatgggt
      721 gcatattaca ctttcacttt aaacttgaat tttaaaccag aagaaaagaa attaaaaatt
      781 gaaccattaa tgaaatcatt gagagcaaca cccttaactc aaattgctgg acttaaagtt
      841 gttaatgttg aagactacat cgatggaatg tataatatgc caggacaaga cttactaaaa
      901 ttttatttag aagataagtc atgatttgct gttcgcccaa gtggaactga acctaaacta
      961 aaaatttatt ttata
//
