LOCUS       sequence-151263-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
       61 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
      121 gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg
      181 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
      241 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
      301 gtcgtgggaa ccgccctgat caaaaagtac cctgcgctgg aaagcgagtt cgtgtacggc
      361 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
      421 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
      481 ctggccaacg gcgagatccg gaaggcgcct ctgatcgaga caaacggcga aaccggggag
      541 atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa
      601 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg
      661 cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac
      721 ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
      781 gg
//
