LOCUS       sequence-151269-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRFP1 expressed behind an AHL-inducible construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: pLux02
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             162..173
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    236..257
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(259..281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      296..382
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accatagaat gaggataaat gatcgcgagt aaataatatt cacaatgtac cattttagtc
       61 atatcagata agcattgatt aatatcatta ttgcttctac aagctttaat tttattaatt
      121 attctgtatg tgtcgtcggc atttatgttt ttcatctagt atttctcctc tttctctagt
      181 agtgctcagt atctctatca ctgataggga tgtcaatctc tatcactgat agggactcta
      241 gaagcggccg cgaattctcc tgtgtgaaat tgttatccgc tttaattaaa ggcatcaaat
      301 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      361 cgctctcctg agtaggacaa atccgccgcc ctagacagct gggcgcgccc cccctacggg
      421 cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc cgtggatatg
      481 tggacgatgg ccgcgagcgg ccaccggctg gctcgc
//
