LOCUS       sequence-151271-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mycobacterium tuberculosis MscL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC
  TITLE     Structure of the MscL homolog from Mycobacterium tuberculosis: a 
            gated mechanosensitive ion channel.
  JOURNAL   Science. 1998 Dec 18;282(5397):2220-6.
  PUBMED    9856938
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-12-18"; volume: "282(5397)"; pages: "2220-6"
COMMENT     Sequence Label: MtMscL
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     282..301
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     385..404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        385..403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    404..428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             443..465
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             478..507
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             523..537
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
       61 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag
      121 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
      181 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
      241 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
      301 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
      361 atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
      421 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac catgggccat
      481 catcatcatc atcatcatca tcatcacagc agcggccata tcgacgacga cgacaagcat
      541 atgctcaaag gattcaagga gtttctcgcg cggggtaata tcgtcgacct ggctgtcgcg
      601 gtggtaatcg gcacagcgtt cacggcgttg gtcaccaagt tcaccgacag catcattacg
      661 ccgctgatca accggatcgg cgtcaacgca cagtccgacg tcggcatctt gcggatcggt
      721 atcggcggtg gtcagaccat tgacttgaac gtcttgttgt cggcagcgat caactttttc
      781 ctgatcgcgt tcgcggtgta cttcctagtc gtgctgccct acaacacact acgcaagaag
      841 ggggaggtcg agcagccggg cgacacccaa gtcgtgctgc tcaccgaaat ccgcgatctg
      901 ctcgcgcaaa cgaacgggga ctcgccgggg aggcacggcg gccgtgggac accatcgcca
      961 accgacgggc ctcgcgcgag cacagaatcg caatagcccg acactcgatc cggatccggc
     1021 tgctaacaaa gcccgaaag
//
