LOCUS       sequence-151275-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mycobacterium tuberculosis MscL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC
  TITLE     Structure of the MscL homolog from Mycobacterium tuberculosis: a 
            gated mechanosensitive ion channel.
  JOURNAL   Science. 1998 Dec 18;282(5397):2220-6.
  PUBMED    9856938
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-12-18"; volume: "282(5397)"; pages: "2220-6"
COMMENT     Sequence Label: MtMscL
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(422..427,428..444,445..450)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(625..672)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     668..686
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacctc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattctcatg
      421 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac
      481 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      541 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
      601 tccggatata gttcctcctt tcagcaaaaa acccctcaag acccgtttag aggccccaag
      661 gggttatgct agttattgct cagcggtggc agcagccaac tcagcttcct ttcgggcttt
      721 gttagcagcc ggatccggat cgagtgtcgg gctattgcga ttctgtgctc gcgcgaggcc
      781 cgtcggttgg cgatggtgtc ccacggccgc cgtgcctccc cggcgagtcc ccgttcgttt
      841 gcgcgagcag atcgcggatt tcggtgagca gcacgacttg ggtgtcgccc ggctgctcga
      901 cctccccctt cttgcgtagt gtgttgtagg gcagcacgac taggaagtac accgcgaacg
      961 cgatcaggaa aaagttgatc gctgccgaca acaagacgtt caagtcaatg gtctga
//
