LOCUS       sequence-151281-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB085d6
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(93..110)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      263..349
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      441..468
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaacg ccatgggcat
       61 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      121 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
      181 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
      241 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      301 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
      361 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
      421 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
      481 aactcgctga gggttctaga
//
