LOCUS       sequence-151282-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of plasma membrane signal from Lyn11 fused to CIBN and 
            tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
  TITLE     Optogenetic control of phosphoinositide metabolism.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 
            10.1073/pnas.1211305109. Epub 2012 Jul 30.
  PUBMED    22847441
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211305109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-08-28- 28"; volume: "109"; issue: "35"; pages: 
            "E2316-23"
COMMENT     Sequence Label: Lyn11-CIBN-GFP
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..594
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(655..676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(916..935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc accatgggat gtattaaatc aaaaaggaaa
       61 gacgggagcg ctagcgccgc caccatgaat ggagctatag gaggtgacct tttgctcaat
      121 tttcctgaca tgtcggtcct agagcgccaa agggctcacc tcaagtacct caatcccacc
      181 tttgattctc ctctcgccgg cttctttgcc gattcttcaa tgattaccgg cggcgagatg
      241 gacagctatc tttcgactgc cggtttgaat cttccgatga tgtacggtga gacgacggtg
      301 gaaggtgatt caagactctc aatttcgccg gaaacgacgc ttgggactgg aaatttcaag
      361 gcagcgaagt ttgatacaga gactaaggat tgtaatgagg cggcgaagaa gatgacgatg
      421 aacagagatg acctagtaga agaaggagaa gaagagaagt cgaaaataac agagcaaaac
      481 aatgggagca caaaaagcat caagaagatg aaacacaaag ccaagaaaga agagaacaat
      541 ttctctaatg attcatctaa agtgacgaag gaattggaga aaacggatta tattcatgta
      601 ccggtcgcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1021 ctggggcaca agctggagta caactacaac agccacaacg tcta
//
