LOCUS       sequence-151283-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of plasma membrane signal from Lyn11 fused to CIBN and 
            tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P
  TITLE     Optogenetic control of phosphoinositide metabolism.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 
            10.1073/pnas.1211305109. Epub 2012 Jul 30.
  PUBMED    22847441
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211305109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-08-28- 28"; volume: "109"; issue: "35"; pages: 
            "E2316-23"
COMMENT     Sequence Label: Lyn11-CIBN-GFP
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..547)
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     promoter        complement(630..833)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(659..679)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggcgaccg gtacatgaat ataatccgtt ttctccaatt
       61 ccttcgtcac tttagatgaa tcattagaga aattgttctc ttctttcttg gctttgtgtt
      121 tcatcttctt gatgcttttt gtgctcccat tgttttgctc tgttattttc gacttctctt
      181 cttctccttc ttctactagg tcatctctgt tcatcgtcat cttcttcgcc gcctcattac
      241 aatccttagt ctctgtatca aacttcgctg ccttgaaatt tccagtccca agcgtcgttt
      301 ccggcgaaat tgagagtctt gaatcacctt ccaccgtcgt ctcaccgtac atcatcggaa
      361 gattcaaacc ggcagtcgaa agatagctgt ccatctcgcc gccggtaatc attgaagaat
      421 cggcaaagaa gccggcgaga ggagaatcaa aggtgggatt gaggtacttg aggtgagccc
      481 tttggcgctc taggaccgac atgtcaggaa aattgagcaa aaggtcacct cctatagctc
      541 cattcatggt ggcggcgcta gcgctcccgt ctttcctttt tgatttaata catcccatgg
      601 tgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
      661 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      721 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      781 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      841 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      901 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      961 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc caccc
//
