LOCUS       sequence-151304-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB029f
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(170..187)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(488..511)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
       61 cgagatcacc aaggtagtcg gcaaataaac gccatgggca ttaaaaaatg ccctcttggg
      121 ttatcaagag ggtcattata tttgctgagg agtgccgtta attaagttaa tgatggtgat
      181 gatggtggcg tttcggcgcc ggataatgca gcgtggtgat cagaccatcc gcaaccgtgc
      241 tatggtgatg caccagttct gccagggtat tgaagcgaga ttcactagaa acgtacagtt
      301 tgccatcgct ggcggtgttg atacggtagt gatacacgcg accttcataa cgcagagaaa
      361 tgctgcgctg gcccgggcta ctttcacttt cacgaaccag aaaagaacca ttaatgccag
      421 agctcagcag gtattcggcc gcgttacggc tcacagggcc atgataccag ctgtgttttt
      481 ccagactctt gtcatcgtcg tccttgtagt ccccaaacgt ctcttcaggc cactgactag
      541 cgataacttt ccccacaacg gaacaactct cattgcatgg gatcattggg tactgtgggt
      601 ttagtggttg taaaaacacc tgaccgctat ccctgatcag tttcttgaag gtaaactcat
      661 cacccccaag tctggctatg cagaaatcac ctggctcaac agcctgctca gggtcaacga
      721 gaattaacat tccgtcagga aagcttggct tggagcctgt tggtgcggtc atggaattac
      781 cttcaacctc aagccagaat gcaga
//
