LOCUS       sequence-151305-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB031a
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    361..382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(397..402,403..420,421..427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    435..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
       61 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
      121 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
      181 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacaa
      241 gaggacatcc ggtcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttgc
      301 tgaggagact tagggaccct ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      361 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      421 tatgttgtgt ggaattgtga gcggataaca atttcacaca cctgcaggtg cagtaaggag
      481 gaaaaaaaaa tgggcagctc tgtgagtagc gttccgacca aactggaagt ggttgcagca
      541 accccgacga gcctgctgat ttcttgggat gccccgatgt ctagtagctc tgtgtattac
      601 tatcgtatca cctacggtga aacgggcggt aacagcccgg tgcaggaatt tacggttccg
      661 tatagtagct ctaccgcgac gattagtggc ctgagcccgg gtgtggatta caccatcacg
      721 gtttatgcat ggggcgaaga tagcgcgggt tacatgttca tgtattctcc gattagtatc
      781 aattac
//
