LOCUS       sequence-151306-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB031a
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(463..480)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
       61 accaaggtag tcggcaaata aacgccatgg gcattaaaaa atgccctctt gggttatcaa
      121 gagggtcatt atatttgctg aggagtgccg ttaattaagt tagtccttct gtcttaaatg
      181 gattttggta tgcctcttgc gttcatcact cctggcaaac ttcctcccac aaatgtcaca
      241 ggcaaaaggc ttctcgcctg tgtgggtgcg gatgtgggtg gtaaggtggt cactacgact
      301 gaagttacgc atgcagattc gacactggaa gggcttctgg cctgtgtgga tgcggatatg
      361 gcgggtaagc tcatccgagc gagaaaagcg gcgatcgcag gactcgacag ggcaagcata
      421 tgggcgttca tgggggggtg tcttgctacc gccgctaccg ccatgatggt gatgatggtg
      481 gcaggtgcgg taattgatac taatcggaga atacatgaac atgtaacccg cgctatcttc
      541 gccccatgca taaaccgtga tggtgtaatc cacacccggg ctcaggccac taatcgtcgc
      601 ggtagagcta ctatacggaa ccgtaaattc ctgcaccggg ctgttaccgc ccgtttcacc
      661 gtaggtgata cgatagtaat acacagagct actagacatc ggggcatccc aagaaatcag
      721 caggctcgtc ggggttgctg caaccacttc cagtttggtc ggaacgctac tcacagagct
      781 gcc
//
