LOCUS       sequence-151308-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB043a
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(167..184)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
       61 gatcaccaag gtagtcggca aataaacgcc atgggcatta aaaaatgccc tcttgggtta
      121 tcaagagggt cattatattt gctgaggagt gccgttaatt aagttaatga tggtgatgat
      181 ggtgcttggt gatacgagtc tgcgcgtcct tcagtgcctc atcaacggtt tggcggcccg
      241 acgcggcgtt aataacagcc gtgcgtacgg cataccaaaa agctgacatc tgcggaatgt
      301 tcggcatgat ttcgcctttt tgggcgtttt ccatcgtcgc cgcgatgcgg ggatctttcg
      361 ccagttcttc ctcataactt ttcagcgcaa cagcgcccag tggcttgtcc ttgttcacag
      421 cctccagtcc ttcgtctgtc agcagatagt tttccaggaa ctctttagcc aactctttat
      481 ttggggacgc cgcgttgata ccggcggata agacacctac gaacggtttt gacggctgac
      541 ctttgaaggt cggcaggact gtaacgccgt aattgacttt actggtatca atgttcgacc
      601 acgcccaagg tccgttaatc gtcatggcgg tctcaccttt attaaacgca gcttccgcaa
      661 tgctgtagtc ggtgtctgcg ttcatgtgct tattcttaat cagatcaact aaaaaggtca
      721 gaccggcttt ggcaccggcg ttatcgacgc ctacatcctt aatatcgtat ttgccgtttt
      781 cgtacttaaa cgcataaccg ccatccgccg cgatcagagg ccaggtgaag tagg
//
