LOCUS       sequence-151309-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient and retroviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Srikanth S, Kim KD, Gao Y, Woo JS, Ghosh S, Calmettes G, Paz A, 
            Abramson J, Jiang M, Gwack Y
  TITLE     A large Rab GTPase encoded by CRACR2A is a component of subsynaptic 
            vesicles that transmit T cell activation signals.
  JOURNAL   Sci Signal. 2016 Mar 22;9(420):ra31. doi: 10.1126/scisignal.aac9171.
  PUBMED    27016526
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-03-22"; volume: "9(420):ra31. doi"; pages: " 
            10.1126/scisignal.aac9171"
COMMENT     Sequence Label: MO70-N-Flag-hCRACR2A-isoform-a-T559N
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     179..201
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     217..233
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             275..298
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca
       61 gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc
      121 aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc
      181 cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct
      241 ctaggcgccg gaattagatc gatctctcga gatggactac aaagacgatg acgacaaggc
      301 tgcccctgac gggagggtag tctccagacc ccagagactt ggtcaggggt ctggccaggg
      361 gccaaagggg agtggagcct gcctgcatcc cctggacagc ctggagcaga aggagactca
      421 ggagcaaacg tcgggccagc tagtcatgct gaggaaggca caggagttct ttcagacctg
      481 tgatgctgaa ggcaagggct tcatcgccag gaaggatatg cagaggctgc ataaggagct
      541 accgctcagc ctggaggaac tggaggatgt gtttgatgcc ctggatgctg atggcaatgg
      601 ctatctgacc ccacaggagt tcactactgg atttagtcac ttcttcttca gccagaataa
      661 cccaagtcag gaagatgcag gtgaacaggt ggcccagcgc catgaagaga aggtgtatct
      721 gtccagaggg gatgaggatc tgggcgacat gggcgaagat gaggaagccc agttccggat
      781 gctgatggac agacttggag cccaaaaggt gttggaagat gaaagtgatg tcaagcagct
      841 ctggttgcag ctgaagaagg aggaacctca tttactgtcc aactttgaag acttcctgac
      901 cagaatcatc tcccagctcc aagaagccca tgaggagaag aatgaactgg agtgtgccct
      961 aaaaaggaaa attgctgctt atgatgaaga aatccagcat ctctatgagg agatggaaca
     1021 acaaatcaaa
//
