LOCUS       sequence-151316-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB061i
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             482..754
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             764..793
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
       61 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
      121 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
      181 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacaag
      241 aggacatccg gtcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttgct
      301 gaggattcta gagcacagct aacaccacgt cgtccctatc tgctgcccta ggtctatgag
      361 tggttgctgg ataactttac gggcatgcat aaggctcggt atctatattc agggagacca
      421 caacggtttc cctctacaaa taattttgtt taacttttac tagagtaagg aggaaaaaaa
      481 aatggcacgc gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt
      541 actggtcgcc gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc
      601 ggaagaaaac gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa
      661 caaccagatc ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt
      721 acaagccgtt accgctattg ctgaaggtcg tcgtgcggcc gcggaacaaa agcttatttc
      781 tgaagaggac ttgg
//
