LOCUS       sequence-151317-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB061i
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(471..500)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(510..782)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 gagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
       61 agatcaccaa ggtagtcggc aaataaacgc catgggcatt aaaaaatgcc ctcttgggtt
      121 atcaagaggg tcattatatt tgctgaggag tgccgttaat taagttagca ggtgcggtaa
      181 ttgatactaa tcggagaata catgaacatg taacccgcgc tatcttcgcc ccatgcataa
      241 accgtgatgg tgtaatccac acccgggctc aggccactaa tcgtcgcggt agagctacta
      301 tacggaaccg taaattcctg caccgggctg ttaccgcccg tttcaccgta ggtgatacga
      361 tagtaataca cagagctact agacatcggg gcatcccaag aaatcagcag gctcgtcggg
      421 gttgctgcaa ccacttccag tttggtcgga acgctactca cagagctgcc caagtcctct
      481 tcagaaataa gcttttgttc cgcggccgca cgacgacctt cagcaatagc ggtaacggct
      541 tgtaattcag cggcttcctg ctcttgctgt tcctggcgtt cgcgaacgtc gaggatctgg
      601 ttgttgatca gaccttcttc gatttcgcgc agcgcgatta cagtggtttt atcgttttct
      661 tccggtacca gcggatcctt tccgcctacc tgcatctgac gagcgcgacg cgcggcgacc
      721 agtaccaggt caaaacggtt accaattttc tctacagcgt cctgaacagt tacgcgtgcc
      781 attt
//
