LOCUS       sequence-151320-       10127 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding shRNA and mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pFUChW nontargeting shRNA
FEATURES             Location/Qualifiers
     source          1..10127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(107..154)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(202..531)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      216..351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(270..289)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     516..536
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(545..973)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(655..676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     867..886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1019..1243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1232..1249
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1266..1446
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1951..1967
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1952..1968
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1971..2559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2077..2088
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2486..2506
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2586..3296)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2864..2883)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3133..3151
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3293..3302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3409..3428)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        complement(4077..4476)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     promoter        4504..4717
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4638..4657
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    4830..4947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5245..5289)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5474..5707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6200..6325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6372..6552
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6570..6768)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6595..6615)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6770..7149
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7321..7340
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7415..7519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7520..7588,7589..8380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7738..7757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8551..9139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9040..9059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9293..9310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9427..9448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9463..9468,9469..9486,9487..9493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9501..9517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9506..9528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9525..9541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9525..9541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9590..9711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9601..9620)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9655..9674
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(9841..10127,1..88))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc gcggtgagca
       61 ccggaacggc actggtcaac ttggccatgg tttagttcct caccttgtcg tattatacta
      121 tgccgatata ctatgccgat gattaattgt caacacgtgc tgatcagatc cgaaaatgga
      181 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
      241 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
      301 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
      361 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
      421 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
      481 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
      541 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
      601 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
      661 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
      721 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
      781 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
      841 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
      901 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
      961 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
     1021 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     1081 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     1141 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     1201 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg
     1261 gtttctgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     1321 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     1381 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     1441 agacccagta cagtccggat gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc
     1501 aaacctccac tctaatactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     1561 taacaagcgg gtgttctctc cttcattggc ttcttctacc ttctcttgct caactggtac
     1621 tagcttgtag caccatccaa aggtcagtgg atatctgatc cctggccctg gtgtgtagtt
     1681 ctgccaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     1741 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     1801 tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac
     1861 ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct
     1921 acttgtgatt gctccatgtt tttctaggtc tcgaggtcga cggtatcgat gcggggaggc
     1981 ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag
     2041 agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca
     2101 gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga
     2161 tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca
     2221 gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
     2281 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
     2341 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
     2401 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
     2461 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
     2521 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa
     2581 ttgctttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
     2641 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg
     2701 ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg
     2761 tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg
     2821 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
     2881 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
     2941 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     3001 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
     3061 ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc
     3121 agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc
     3181 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
     3241 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg
     3301 gcgaccggta ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa
     3361 aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg
     3421 gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac
     3481 ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc
     3541 cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca
     3601 ccctgcatta taagccaaca gaacgggnna cgtcacgaca cgacgagggc gcgcgctccc
     3661 aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac
     3721 aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc
     3781 ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt
     3841 gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct
     3901 tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag
     3961 ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca cgcttccgtc
     4021 ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg ctactcacca
     4081 agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc tgcgacggaa
     4141 ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg ccccacggag
     4201 atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg ctctctggaa
     4261 agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc tgctgatact
     4321 ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc cgggcggaag
     4381 gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg aggagggggg
     4441 cgcccgcggg aggcgccaaa acccggcgcg gaggccagat ctggagccga cacgggttaa
     4501 ttaaacgctg acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg
     4561 aacacccagc gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc
     4621 cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt
     4681 tggatttggg aatcttataa gttctgtatg agaccacagg gatccgacta gaaggcacag
     4741 agggattcaa gagatccctc tgtgccttct agtctttttg aattcttaat taaccaaact
     4801 ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     4861 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     4921 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tctgcagaat
     4981 tggcgcacgc agtgccgatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
     5041 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
     5101 aaacgataat ggtgaatatc cctgcctaac tctattcact atacaaagta cagcaaaaac
     5161 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     5221 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     5281 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     5341 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     5401 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     5461 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     5521 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     5581 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     5641 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     5701 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     5761 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     5821 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     5881 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     5941 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     6001 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     6061 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     6121 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     6181 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     6241 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     6301 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     6361 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     6421 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     6481 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     6541 ccagagagac ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc
     6601 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     6661 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     6721 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     6781 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     6841 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     6901 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     6961 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     7021 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     7081 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     7141 atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     7201 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     7261 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     7321 cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc
     7381 gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8041 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9301 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9361 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9421 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9481 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9541 ccatgattac gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt
     9601 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     9661 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc
     9721 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
     9781 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg
     9841 tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
     9901 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
     9961 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
    10021 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
    10081 gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccggg
//
