LOCUS       sequence-151321-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB061i10
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(472..501)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(511..783)
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 agagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
       61 gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc cctcttgggt
      121 tatcaagagg gtcattatat ttgctgagga gtgccgttaa ttaagttagc aggtgcggta
      181 attgatacta atcggagaat acatgaacat acgacccgcg ctatcttcgc cccatgcata
      241 aaccgtgatg gtgtaatcca cacccgggct caggccacta atcgtcgcgg tagagctact
      301 atacggaacc gtaaattcct gcaccgggct gttaccgccc gtttcaccgt aggtgatacg
      361 atagtaatac acagagctac tagacatcgg ggcatcccaa gaaatcagca ggctcgtcgg
      421 ggttgctgca accacttcca gtttggtcgg aacgctactc acagagctgc ccaagtcctc
      481 ttcagaaata agcttttgtt ccgcggccgc acgacgacct tcagcaatag cggtaacggc
      541 ttgtaattca gcggcttcct gctcttgctg ttcctggcgt tcgcgaacgt cgaggatctg
      601 gttgttgatc agaccttctt cgatttcgcg cagcgcgatt acagtggttt tatcgttttc
      661 ttccggtacc agcggatcct ttccgcctac ctgcatctga cgagcgcgac gcgcggcgac
      721 cagtaccagg tcaaaacggt taccaatttt ctctacagcg tcctgaacag ttacgcgtgc
      781 catttt
//
