LOCUS       sequence-151322-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient and retroviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Srikanth S, Kim KD, Gao Y, Woo JS, Ghosh S, Calmettes G, Paz A, 
            Abramson J, Jiang M, Gwack Y
  TITLE     A large Rab GTPase encoded by CRACR2A is a component of subsynaptic 
            vesicles that transmit T cell activation signals.
  JOURNAL   Sci Signal. 2016 Mar 22;9(420):ra31. doi: 10.1126/scisignal.aac9171.
  PUBMED    27016526
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-03-22"; volume: "9(420):ra31. doi"; pages: " 
            10.1126/scisignal.aac9171"
COMMENT     Sequence Label: MO70-N-Flag-hCRACR2A-isoform-a-Q604L
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     178..200
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     216..232
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             274..297
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
       61 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
      121 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
      181 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      241 taggcgccgg aattagatcg atctctcgag atggactaca aagacgatga cgacaaggct
      301 gcccctgacg ggagggtagt ctccagaccc cagagacttg gtcaggggtc tggccagggg
      361 ccaaagggga gtggagcctg cctgcatccc ctggacagcc tggagcagaa ggagactcag
      421 gagcaaacgt cgggccagct agtcatgctg aggaaggcac aggagttctt tcagacctgt
      481 gatgctgaag gcaagggctt catcgccagg aaggatatgc agaggctgca taaggagcta
      541 ccgctcagcc tggaggaact ggaggatgtg tttgatgccc tggatgctga tggcaatggc
      601 tatctgaccc cacaggagtt cactactgga tttagtcact tcttcttcag ccagaataac
      661 ccaagtcagg aagatgcagg tgaacaggtg gcccagcgcc atgaagagaa ggtgtatctg
      721 tccagagggg atgaggatct gggcgacatg ggcgaagatg aggaagccca gttccggatg
      781 ctgatggaca gacttggagc ccaaaaggtg ttggaagatg aaagtgatgt caagcagctc
      841 tggttgcagc tgaagaagga ggaacctcat ttactgtcca actttgaaga cttcctgacc
      901 agaatcatct cccagctcca agaagcccat gaggagaaga atgaactgga gtgtgcccta
      961 aaaaggaaaa ttgctgctta tgatgaagaa atccagcatc tctatgagga gatggaacaa
     1021 caaatcaaaa g
//
