LOCUS       sequence-151331-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Directed Evolution of Protein-Protein Interactions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance 
            AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
  TITLE     Continuous evolution of Bacillus thuringiensis toxins overcomes 
            insect resistance.
  JOURNAL   Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 
            2016 Apr 27.
  PUBMED    27120167
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17938"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "58-63"
COMMENT     Sequence Label: pAB061i8
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             504..776
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             786..815
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
       61 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
      121 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
      181 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
      241 cgggttactg atgatgaaca agaggacatc cggtcaaata aaacgaaagg ctcagtcgaa
      301 agactgggcc tttcgttttg ctgaggattc tagagcacag ctaacaccac gtcgtcccta
      361 tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc ataaggctcg
      421 gtatctatat tcagggagac cacaacggtt tccctctaca aataattttg tttaactttt
      481 actagagtaa ggaggaaaaa aaaatggcac gcgtaactgt tcaggacgct gtagagaaaa
      541 ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag
      601 gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg
      661 aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc
      721 aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgtgcgg
      781 ccgcggaaca aaagcttatt tctgaagagg acttgggcag ctctgtgagt agcgttccga
      841 ccaaactgga agtggttgca gcaacc
//
