LOCUS       sequence-151341-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression and retroviral Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Srikanth S, Jung HJ, Ribalet B, Gwack Y
  TITLE     The intracellular loop of Orai1 plays a central role in fast 
            inactivation of Ca2+ release-activated Ca2+ channels.
  JOURNAL   J Biol Chem. 2010 Feb 12;285(7):5066-75. doi: 
            10.1074/jbc.M109.072736. Epub 2009 Dec 10.
  PUBMED    20007711
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.072736"; journalName: "J Biol Chem"; date: 
            "2010-02-12- 12"; volume: "285"; issue: "7"; pages: "5066-75"
COMMENT     Sequence Label: MO70 - Orai1 EC-Flag
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     183..205
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     221..237
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      568..577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             906..929
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttacgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
       61 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
      121 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
      181 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
      241 ttctctaggc gccggaatta gatcgatctc tcgagatgca tccggagccc gccccgagcc
      301 gtagcagtcc cgagcttccc ccaagcggcg gcagcaccac cagcggcagc cgccggagcc
      361 gccgccgcag cggggacggg gagcccccgg gggccccgcc accgccgccg tccgccgtca
      421 cctacccgga ctggatcggc cagagttact ccgaggtgat gagcctcaac gagcactcca
      481 tgcaggcgct gtcctggcgc aagctctact tgagccgcgc caagcttaaa gcctccagcc
      541 ggacctcggc tctgctctcc ggcttcgcca tggtggcaat ggtggaggtg cagctggacg
      601 ctgaccacga ctacccaccg gggctgctca tcgccttcag tgcctgcacc acagtgctgg
      661 tggctgtgca cctgtttgcg ctcatgatca gcacctgcat cctgcccaac atcgaggcgg
      721 tgagcaacgt gcacaatctc aactcggtca aggagtcccc ccatgagcgc atgcaccgcc
      781 acatcgagct ggcctgggcc ttctccaccg tcattggcac gctgctcttc ctagctgagg
      841 tggtgctgct ctgctgggtc aagttcttgc ccctcaagaa gcagccagtc gacggcagcg
      901 gcagcgacta caaggacgac gatgacaagg gcagcggcag cacgcgtggc cagccaaggc
      961 ccaccagcaa g
//
