LOCUS       sequence-151343-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes a toxin gene (relE) under the control
            of rhamnose induceable promoter (PrhaB) to be used as a negative 
            selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-217
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(180..426)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     promoter        complement(797..915)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
ORIGIN
        1 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga attagccatg
       61 gtccatatga atatcctcct tagttcctat tccgaagttc ctattctcta gaaagtatag
      121 gaacttcaga gcgatcctga cgtccccatc cagtgcaaag ctagcttctt cgtctgtttc
      181 tactggtatt ggcacaaacc tgattccaat ttgagcaagg ctatgtgcca tctcgatact
      241 cgttcttaac tcaacagaag atgctttgtg catacagccc ctcgtttatt atttatctcc
      301 tcagccagcc gctgtgcttt cagtggattt cggataacag aaaggccggg aaatacccag
      361 cctcgctttg taacggagta gagacgaaag tgattgcgcc tacccggata ttatcgtgag
      421 gatgcgtcat cgccattaat tcactgatca gtgataagct gtcaaacatg agaattcgcg
      481 gatcctcaga gaatgcgttt gaccgcctcg ctatatactt ccgagcgttc tcttttccca
      541 acagaaatca cgaaaacgac aactttctcg tctataacct ggtatacaag gcgatagcct
      601 gaagaccgga gcttaatctt gtaacaatca ggcataccac ggagcttgtt tgcttcaatc
      661 cggggtgact caagtacttc aaccagcttc tttttcaact gttcacgtac cgtcgagccc
      721 agctttcgcc attcctttag tgcccgctcg tcaaaatcca gaaaatacgc catatggtga
      781 tcctgctgaa tttcattacg accagtctaa aaagcgcctg aattcgcgac cttctcgtta
      841 ctgacaggaa aatgggccat tggcaaccag ggaaagatga acgtgatgat gttcacaatt
      901 tgctgaattg tggtgatgtg atgctcaccg catttcctga aaattcacgc tgtatcttga
      961 aaaa
//
