LOCUS       sequence-151355-         472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase
            mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Florea M, Hagemann H, Santosa G, Abbott J, Micklem CN, 
            Spencer-Milnes X, de Arroyo Garcia L, Paschou D, Lazenbatt C, Kong 
            D, Chughtai H, Jensen K, Freemont PS, Kitney R, Reeve B, Ellis T
  TITLE     Engineering control of bacterial cellulose production using a 
            genetic toolkit and a new cellulose-producing strain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):E3431-40. doi: 
            10.1073/pnas.1522985113. Epub 2016 May 31.
  PUBMED    27247386
REFERENCE   2  (bases 1 to 472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1522985113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-14- 14"; volume: "113"; issue: "24"; pages: 
            "E3431-40"
COMMENT     Sequence Label: J-sRNA-331Bb
FEATURES             Location/Qualifiers
     source          1..472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             162..173
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    219..240
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(242..264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      279..365
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 accatagaat gaggataaat gatcgcgagt aaataatatt cacaatgtac cattttagtc
       61 atatcagata agcattgatt aatatcatta ttgcttctac aagctttaat tttattaatt
      121 attctgtatg tgtcgtcggc atttatgttt ttcatctagt atttctcctc tttctctagt
      181 aggcgctatc atgccatacc gcgaaaggtt ttgcaccact ctagaagcgg ccgcgaattc
      241 tcctgtgtga aattgttatc cgctttaatt aaaggcatca aataaaacga aaggctcagt
      301 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      361 caaatccgcc gccctagaca gctgggcgcg ccccccctac gggcttgctc tccgggcttc
      421 gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat atgtggacga tg
//
