LOCUS       sequence-151370-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (chpB) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-238
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..128
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(181..427)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 ctctcaaagc aattttgagt gacacaggaa cacttaacgg ctgacatggg aattagccat
       61 ggtccatatg aatatcctcc ttagttccta ttccgaagtt cctattctct agaaagtata
      121 ggaacttcag agcgatcctg acgtccccat ccagtgcaaa gctagcttct tcgtctgttt
      181 ctactggtat tggcacaaac ctgattccaa tttgagcaag gctatgtgcc atctcgatac
      241 tcgttcttaa ctcaacagaa gatgctttgt gcatacagcc cctcgtttat tatttatctc
      301 ctcagccagc cgctgtgctt tcagtggatt tcggataaca gaaaggccgg gaaataccca
      361 gcctcgcttt gtaacggagt agagacgaaa gtgattgcgc ctacccggat attatcgtga
      421 ggatgcgtca tcgccattaa ttcactgatc agtgataagc tgtcaaacat gagaattcgc
      481 ggatccttat tccaccaccg cctgcaagcg taataacgcc tcttccacca cctcatccgc
      541 agccagacca atacgctttg ccagccgggc gtgtagatcc atcatccgca cctgattcac
      601 cagcaccacg ccgtgcacat cgccttcttc gcaatgtaaa ggaacgctaa atccggcata
      661 acgggcaaaa tttccgccct gcgtaatggg ggccaccagc gtcattccca gttgattaaa
      721 ggcttgaacg gagagcacaa gcgcaggtcg accagcacct tgctgttcat ggccgcttgc
      781 tggatcaaag ccaaccagca caatgtctcc ccgttcaaat tcacttttct ttaccatatg
      841 gtgatcctgc tgaatttcat tacgaccagt ctaaaaagcg cctgaattcg cgaccttctc
      901 gttactgaca gg
//
