LOCUS       sequence-151375-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (yhaV) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-239
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..127
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(180..426)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga attagccatg
       61 gtccatatga atatcctcct tagttcctat tccgaagttc ctattctcta gaaagtatag
      121 gaacttcaga gcgatcctga cgtccccatc cagtgcaaag ctagcttctt cgtctgtttc
      181 tactggtatt ggcacaaacc tgattccaat ttgagcaagg ctatgtgcca tctcgatact
      241 cgttcttaac tcaacagaag atgctttgtg catacagccc ctcgtttatt atttatctcc
      301 tcagccagcc gctgtgcttt cagtggattt cggataacag aaaggccggg aaatacccag
      361 cctcgctttg taacggagta gagacgaaag tgattgcgcc tacccggata ttatcgtgag
      421 gatgcgtcat cgccattaat tcactgatca gtgataagct gtcaaacatg agaattcgcg
      481 gatcctcaat gggtttcttc tgtttctcgg gtgagggttt cccagtcggc aggaggatgt
      541 cctcttttta acattttgct gaatacggta taggcatctg tttttttacc gtaggtgcgc
      601 agagtgtttt catcgttcat ccatcccaga atgatgactt tctctttttc actataacga
      661 aagaagagac gataacgacc agcaccaaat tttacccgtg accagtcttt atttttccca
      721 gagcctaacg acttgccatg acggaatgcc ggtgatgatg gattgaccgt gatatgctcc
      781 tcaatcactt tatggactac cgccaataac tttgtggcgg cttttctctg ataattttca
      841 ggatcttttc cctttaatgt ctcgacttcg gcaactaaag cgtcgtaggt ttcctgaaaa
      901 cagggatgag catatagcgc cca
//
