LOCUS       sequence-151397-        8567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 960 interrupted CUG repeats in the context of human DMPK 
            genomic segment containing exons 11-15 with repeats inserted at site
            in exon 15 that contains the repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
  TITLE     Muscleblind proteins regulate alternative splicing.
  JOURNAL   EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
  PUBMED    15257297
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul 
            15"
COMMENT     Sequence Label: DT960
FEATURES             Location/Qualifiers
     source          1..8567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        139..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     597..617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        complement(6242..6260)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6243..6260)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6341..6360)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6460..6482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6520..6538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6606..6710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6711..6779,6780..7571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6929..6948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7742..8330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8484..8501
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
       61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
      721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
      781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
      841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
      901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
      961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
     1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
     1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
     1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
     1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
     1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
     1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
     1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
     1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
     1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
     1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
     1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
     1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
     1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
     1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
     1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
     1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
     1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
     2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
     2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
     2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
     2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
     2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gactgctgct gctgctgctg
     2341 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     2401 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     2461 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     2521 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     2581 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     2641 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2701 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     2761 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     2821 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     2881 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     2941 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     3001 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     3061 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
     3121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     3181 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     3241 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     3301 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     3361 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     3421 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3481 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     3541 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     3601 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     3661 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     3721 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     3781 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     3841 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
     3901 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     3961 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     4021 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     4081 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     4141 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     4201 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     4261 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     4321 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     4381 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     4441 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     4501 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     4561 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     4621 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
     4681 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     4741 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     4801 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     4861 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     4921 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     4981 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     5041 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     5101 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     5161 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     5221 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     5281 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     5341 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     5401 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gacgggggat cacagaccat
     5461 ttctttcttt cggccaggct gaggccctga cgtggatggg caaactgcag gcctgggaag
     5521 gcagcaagcc gggccgtccg tgttccatcc tccacgcacc cccacctatc gttggttcgc
     5581 aaagtgcaaa gctttcttgt gcatgacgcc ctgctctggg gagcgtctgg cgcgatctct
     5641 gcctgcttac tcgggaaatt tgcttttgcc aaacccgctt tttcggggat cccgcgcccc
     5701 cctcctcact tgcgctgctc tcggagcccc agccggctcc gcccgcttcg gcggtttgga
     5761 tatttattga cctcgtcctc cgactcgctg acaggctaca ggacccccaa caaccccaat
     5821 ccacgttttg gatgcactga gaccccgaca ttcctcggta tttattgtct gtccccacct
     5881 aggaccccca cccccgaccc tcgcgaataa aaggccctcc atctgcccaa agctctggac
     5941 tccacagtgt ccgcggtttg cgttgtgggc cggagctccg cagcgggcca atccggaggc
     6001 gtgtggaggc ggccgaaggt ctgggaggag ctagcgggat gcgaagcggc cgaatcaggg
     6061 ttgggggagg aaaagccacg gggcggggct ttggcgtccg gccaatagga gggcgagcgg
     6121 gccacccgga ggcaccgccc ccgcccagct gtggcccagc tgtgccaccg agcgtcgaga
     6181 agagggggct gggctggcag cgcgcgcggc catcctcctt ccactgcgcc tgcgctcgag
     6241 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     6301 cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc tctgatgccg
     6361 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6421 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6481 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6541 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6601 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6661 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6721 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6781 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     6841 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     6901 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     6961 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7021 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7081 ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7141 aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7201 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7261 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7321 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7381 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7441 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca
     7501 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7561 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7621 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7681 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7741 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7801 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7861 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     7921 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7981 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8041 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8101 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     8161 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8221 agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8281 ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca
     8341 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8401 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8461 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8521 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
