LOCUS       sequence-151398-         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: contains 5-state register that regulates expression 
            of GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR187
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     468..496
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     RBS             532..540
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
       61 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      121 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      181 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      241 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
      301 ctgggtatct cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt gttggccatg
      361 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      421 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
      481 caagaattgg gacaactcca gtgaaaagtt cttctccttt actcattagt gacctcctta
      541 atataggtcg gcttcgacct taaacaa
//
