LOCUS       sequence-151399-        7017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 480 interrupted CUG repeats in the context of human DMPK 
            genomic segment containing exons 11-15 with repeats inserted at site
            in exon 15 that contains the repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
  TITLE     Muscleblind proteins regulate alternative splicing.
  JOURNAL   EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
  PUBMED    15257297
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul 
            15"
COMMENT     Sequence Label: DT480
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        139..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     597..617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        complement(4692..4710)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4693..4710)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4791..4810)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4910..4932
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4970..4988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5056..5160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5161..5229,5230..6021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5379..5398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6192..6780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6934..6951
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
       61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
      721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
      781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
      841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
      901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
      961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
     1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
     1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
     1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
     1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
     1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
     1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
     1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
     1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
     1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
     1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
     1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
     1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
     1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
     1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
     1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
     1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
     1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
     2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
     2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
     2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
     2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
     2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gactgctgct gctgctgctg
     2341 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     2401 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     2461 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     2521 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     2581 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     2641 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     2701 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     2761 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     2821 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     2881 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     2941 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     3001 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     3061 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
     3121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     3181 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
     3241 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
     3301 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
     3361 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     3421 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
     3481 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
     3541 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
     3601 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
     3661 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
     3721 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
     3781 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
     3841 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gatcgacggg ggatcacaga
     3901 ccatttcttt ctttcggcca ggctgaggcc ctgacgtgga tgggcaaact gcaggcctgg
     3961 gaaggcagca agccgggccg tccgtgttcc atcctccacg cacccccacc tatcgttggt
     4021 tcgcaaagtg caaagctttc ttgtgcatga cgccctgctc tggggagcgt ctggcgcgat
     4081 ctctgcctgc ttactcggga aatttgcttt tgccaaaccc gctttttcgg ggatcccgcg
     4141 cccccctcct cacttgcgct gctctcggag ccccagccgg ctccgcccgc ttcggcggtt
     4201 tggatattta ttgacctcgt cctccgactc gctgacaggc tacaggaccc ccaacaaccc
     4261 caatccacgt tttggatgca ctgagacccc gacattcctc ggtatttatt gtctgtcccc
     4321 acctaggacc cccacccccg accctcgcga ataaaaggcc ctccatctgc ccaaagctct
     4381 ggactccaca gtgtccgcgg tttgcgttgt gggccggagc tccgcagcgg gccaatccgg
     4441 aggcgtgtgg aggcggccga aggtctggga ggagctagcg ggatgcgaag cggccgaatc
     4501 agggttgggg gaggaaaagc cacggggcgg ggctttggcg tccggccaat aggagggcga
     4561 gcgggccacc cggaggcacc gcccccgccc agctgtggcc cagctgtgcc accgagcgtc
     4621 gagaagaggg ggctgggctg gcagcgcgcg cggccatcct ccttccactg cgcctgcgca
     4681 cttaatcgag ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     4741 taattgtagc cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc
     4801 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4861 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4921 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5401 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5521 tgcagtgctg ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc
     5581 ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt
     5641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5701 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5941 acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6301 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6541 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     6601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6661 cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa
     6781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6961 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
