LOCUS       sequence-151402-        5577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 40 interrupted CUG repeats in the context of human DMPK 
            genomic segment containing exons 11-15 with repeats inserted at site
            in exon 15 that contains the repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
  TITLE     Muscleblind proteins regulate alternative splicing.
  JOURNAL   EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
  PUBMED    15257297
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul 
            15"
COMMENT     Sequence Label: DT40
FEATURES             Location/Qualifiers
     source          1..5577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        139..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     597..617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        complement(3252..3270)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3253..3270)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3351..3370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3470..3492
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3530..3548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3616..3720
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3721..3789,3790..4581)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3939..3958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4752..5340
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5494..5511
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
       61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
      721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
      781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
      841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
      901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
      961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
     1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
     1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
     1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
     1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
     1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
     1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
     1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
     1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
     1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
     1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
     1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
     1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
     1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
     1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
     1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
     1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
     1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
     2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
     2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
     2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
     2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
     2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gactgctgct gctgctgctg
     2341 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
     2401 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gacgggggat
     2461 cacagaccat ttctttcttt cggccaggct gaggccctga cgtggatggg caaactgcag
     2521 gcctgggaag gcagcaagcc gggccgtccg tgttccatcc tccacgcacc cccacctatc
     2581 gttggttcgc aaagtgcaaa gctttcttgt gcatgacgcc ctgctctggg gagcgtctgg
     2641 cgcgatctct gcctgcttac tcgggaaatt tgcttttgcc aaacccgctt tttcggggat
     2701 cccgcgcccc cctcctcact tgcgctgctc tcggagcccc agccggctcc gcccgcttcg
     2761 gcggtttgga tatttattga cctcgtcctc cgactcgctg acaggctaca ggacccccaa
     2821 caaccccaat ccacgttttg gatgcactga gaccccgaca ttcctcggta tttattgtct
     2881 gtccccacct aggaccccca cccccgaccc tcgcgaataa aaggccctcc atctgcccaa
     2941 agctctggac tccacagtgt ccgcggtttg cgttgtgggc cggagctccg cagcgggcca
     3001 atccggaggc gtgtggaggc ggccgaaggt ctgggaggag ctagcgggat gcgaagcggc
     3061 cgaatcaggg ttgggggagg aaaagccacg gggcggggct ttggcgtccg gccaatagga
     3121 gggcgagcgg gccacccgga ggcaccgccc ccgcccagct gtggcccagc tgtgccaccg
     3181 agcgtcgaga agagggggct gggctggcag cgcgcgcggc catcctcctt ccactgcgcc
     3241 tgcgctcgag ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     3301 taattgtagc cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc
     3361 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3421 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3481 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3541 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3721 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3781 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3841 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3901 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3961 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4021 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4081 tgcagtgctg ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc
     4141 ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt
     4201 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4261 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4321 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4381 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4441 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4501 acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4561 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4621 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4681 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4741 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4801 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4861 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4921 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4981 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5041 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5101 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     5161 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5221 cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5281 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa
     5341 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5401 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5461 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5521 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
