LOCUS       sequence-151406-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TP901 attL-attR. GFP expression will stop if sites recombine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR287
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        192..241
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             274..285
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(322..350)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca tgcataaggc
       61 tcgtataata tattcaggga gaccacaacg gtttccctct acaaataatt ttgtttaact
      121 ttttaattaa cggccgatgc caacacaatt aacatctcaa ttaaggtaac taaaaaactc
      181 cttttgcaca gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca
      241 gcctctacaa ataattttgt ttaagaattc attaaagagg agaaaggtac catgagtaaa
      301 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      361 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      421 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      481 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      541 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      601 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      661 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      721 aactataact cacacaatgt atacatcatg gcagacaa
//
