LOCUS       sequence-151407-        5447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 0 CUG repeats in the context of human DMPK genomic segment
            containing exons 11-15 with repeats inserted at site in exon 15 that
            contains the repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
  TITLE     Muscleblind proteins regulate alternative splicing.
  JOURNAL   EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
  PUBMED    15257297
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul 
            15"
COMMENT     Sequence Label: DT0
FEATURES             Location/Qualifiers
     source          1..5447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        139..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     597..617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        complement(3122..3140)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3123..3140)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3221..3240)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3340..3362
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3400..3418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3486..3590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3591..3659,3660..4451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3809..3828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4622..5210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5364..5381
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
       61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
      661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
      721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
      781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
      841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
      901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
      961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
     1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
     1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
     1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
     1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
     1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
     1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
     1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
     1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
     1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
     1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
     1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
     1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
     1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
     1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
     1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
     1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
     1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
     2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
     2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
     2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
     2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
     2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gacgggggat cacagaccat
     2341 ttctttcttt cggccaggct gaggccctga cgtggatggg caaactgcag gcctgggaag
     2401 gcagcaagcc gggccgtccg tgttccatcc tccacgcacc cccacctatc gttggttcgc
     2461 aaagtgcaaa gctttcttgt gcatgacgcc ctgctctggg gagcgtctgg cgcgatctct
     2521 gcctgcttac tcgggaaatt tgcttttgcc aaacccgctt tttcggggat cccgcgcccc
     2581 cctcctcact tgcgctgctc tcggagcccc agccggctcc gcccgcttcg gcggtttgga
     2641 tatttattga cctcgtcctc cgactcgctg acaggctaca ggacccccaa caaccccaat
     2701 ccacgttttg gatgcactga gaccccgaca ttcctcggta tttattgtct gtccccacct
     2761 aggaccccca cccccgaccc tcgcgaataa aaggccctcc atctgcccaa agctctggac
     2821 tccacagtgt ccgcggtttg cgttgtgggc cggagctccg cagcgggcca atccggaggc
     2881 gtgtggaggc ggccgaaggt ctgggaggag ctagcgggat gcgaagcggc cgaatcaggg
     2941 ttgggggagg aaaagccacg gggcggggct ttggcgtccg gccaatagga gggcgagcgg
     3001 gccacccgga ggcaccgccc ccgcccagct gtggcccagc tgtgccaccg agcgtcgaga
     3061 agagggggct gggctggcag cgcgcgcggc catcctcctt ccactgcgcc tgcgctcgag
     3121 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     3181 cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc tctgatgccg
     3241 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3301 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3361 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3421 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3961 ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4021 aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggca
     4381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4741 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4981 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     5041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5101 agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5161 ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa aacgccagca
     5221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5401 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
