LOCUS       sequence-151417-        7582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Loayza D, De Lange T
  TITLE     POT1 as a terminal transducer of TRF1 telomere length control.
  JOURNAL   Nature. 2003 Jun 26. 423(6943):1013-8.
  PUBMED    12768206
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Jun 26. 423(6943):1013-8."
COMMENT     Sequence Label: pLPC myc hPOT1
FEATURES             Location/Qualifiers
     source          1..7582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(2694..2723)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2723..2732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2763..2782)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2764..2782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2827..3030)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2856..2876)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3031..3410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3582..3601
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3690..4289)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3773..3793)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4270..4289
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4307..4723)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4323..4339)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4355..4377)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4924..5123
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             5188..5657
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5688..5792
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5793..5861,5862..6653)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6011..6030)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6813..7401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7302..7321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7555..7572
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcggccctc tagatgcatg ctcgagttag
      781 attacatctt ctgcaactgt ggtgtcaaaa atctgatagc aaatttgatt atctgttcca
      841 tttgtgacat tgtatgactt gatgaagcat tccaaccacg gatatgcatc aatttttatt
      901 cctggaggac aaaacatatc catgatcata tccacacttt tctgaaggtc atcatccatc
      961 agaacttctg atgctggaat ctggaagaat ttgtcagaat ccatgagata ggcttctagt
     1021 actcctgttc catcatcaag tgtaaaggtc ataacaaaca catattggag gggtacaata
     1081 cccagtgctt ctgccacaga agaaggaatc cacgatgttt tatcaaccag ggaatttaga
     1141 ttttgtatgg atctcaaact agaacactgt ttacatccat agtgatgtat tgttccttgt
     1201 ataagaaatg gtgctgaaag gtccaaaagt tccaggtctt cgtggccaga tctcacagga
     1261 attacactat taaacttgtt cgagagtttg caaatttcac tgagtgtacc tccttctatc
     1321 aaaagtagac attcatttga aagcgggaga ataccattat ttttcacaaa atgaactgct
     1381 acttttcgtc ctttttgatt tttagtggtc cagatttttg aatcatataa tgatgtattt
     1441 tgtagcttga catctggggt tttagttgca ccatcctgaa aaattatatc caaatcgccc
     1501 tcatgtggaa cttcttgcag caaatgacat ttagggcaat gaagtttaac agactgaaat
     1561 agtcttctgg gcttatatga cctcaatttt gctcggatgc ggtattgttg aggagctttt
     1621 tgtttcaaaa tggcacatag tggtgtcctc tccaaatact gatgatctgt aagtattgta
     1681 gcagatagct gttgacatct ttctacctcg tataatgata ctgatccaga gcttggaaag
     1741 ctgtcgtcag gttctgattg acagataaca tctgaatgct gattggctgt caaatttgca
     1801 gattctaaat cctttttcag ttgatccaca tcagagttac tttctggcaa gaccctgatt
     1861 ccccgaccgt aactggtacc tccatgaaga tgaaactcta aacttaacat tgtctgattc
     1921 tctgaattca ttgattgaag tttggtatga aggctataga ttctaagaaa gcttccaacc
     1981 ttcagagatc ttgccacatg aacatggtta tcgtagacta aaatgtctat tgtcagattt
     2041 tgtagccgat ggatgtgact taaatcacct tcaagaacaa ggtcttgtat taagactctc
     2101 caagatggaa atggtgtcct ggtgccatcc cataccttta gaagaaatga tgctccgtcc
     2161 acttctgctt tgcccaagag ctgacaagtc aggtcaaaat actgcattgg ctgaacatca
     2221 cacaatttta gtaatgtcca agacggtgac atatgagtag atgcccaaac acgtaaggct
     2281 tctaccattt tgtggtcctc agtagtgaag ttaaaatact tgcttgaagt gcgaggtatg
     2341 ataggggctc ccaaagttcc ctcaaacgtc aaagatgcaa agccagagct ggtgataccc
     2401 tgagtctcct ttttatatac ttgaatcttc agcctgtgaa agcgaacaat atctccattt
     2461 ttataaatta ttggaagggc ttcatagttt ccactaaaga gcaggcaagt tagttttaca
     2521 tttgtctggt ccacaatagt tacaactgag caataatcag ttcctttgct tagatatggg
     2581 ggcttaaaga acttcacaac accatagaca ttgacaattg taccaccctt aagttgattc
     2641 aggggtgtat atatataatt tgttgctgga accaaggatc ctctctttct taacaaatct
     2701 tcttcagaaa tcaacttttg ttccatggtg gctgatccga gctcggtacc aagcttgggt
     2761 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
     2821 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     2881 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     2941 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     3001 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     3061 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     3121 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     3181 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     3241 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     3301 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     3361 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
     3421 tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca
     3481 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
     3541 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
     3601 actgagagtg caccataggg gatcgggaga tccgatgcat ggggtcgtgc gctcctttcg
     3661 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
     3721 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     3781 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
     3841 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     3901 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     3961 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     4021 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     4081 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     4141 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     4201 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     4261 aggcgcaccg tgggcttgta ctcggtcatg gtaagctgat ccggccggcg cctagagaag
     4321 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
     4381 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
     4441 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
     4501 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
     4561 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
     4621 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
     4681 agtgacctaa ggtcaaactt aagggagtgg taacagtctg gccctaattt tcagacaaat
     4741 acagaaacac agtcagacag agacaacaca gaacgatgct gcagcgctgc agcagacaag
     4801 acgcgcggct tcggttccaa accgaaagca aaaattcaga cggaggcggg aactgtttta
     4861 ggttctcgtc tcctaccaga accacatatc ctgacggggt cggattccac atcgactccc
     4921 ttcctcaggt cgggccacaa aaacggcccc caaagtccct gggacgtctc ccagggttgc
     4981 ggccgggtgt tcagaactcg tcagttccac cacgggtccg ccagatacag agctagttag
     5041 ctaactagta ccgacgcagg cgcataaaat cagtcataga cactagacaa tcggacagac
     5101 acagataagt tgctggccag cttgcctccc ggtggtgggt cggtggtccc tgggcagggg
     5161 tctcccgatc ccggacgagc ccccaaatga aagacccccg ctgacgggta gtcaatcact
     5221 cagaggagac cctcccaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     5281 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     5341 gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc
     5401 gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg
     5461 aaacatctga tggttctcta gaaactgctg agggctggac cgcatctggg gaccatctgt
     5521 tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc
     5581 agctgttcca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     5641 gtttggccca tattcaggct gcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5701 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5761 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5821 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5881 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5941 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6001 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6061 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6121 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6181 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6241 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6301 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6361 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6421 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6481 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6541 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6601 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6661 accaagttta ctcatatata ctttagattg atttgcggcc gcaaaaggat ctaggtgaag
     6721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7561 tcagtgagcg aggaagcgga ag
//
