LOCUS       sequence-151418-        7210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Loayza D, De Lange T
  TITLE     POT1 as a terminal transducer of TRF1 telomere length control.
  JOURNAL   Nature. 2003 Jun 26. 423(6943):1013-8.
  PUBMED    12768206
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Jun 26. 423(6943):1013-8."
COMMENT     Sequence Label: pLPC myc hPOT1 delta OB
FEATURES             Location/Qualifiers
     source          1..7210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(2322..2351)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2351..2360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2391..2410)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2392..2410)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2455..2658)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2484..2504)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2659..3038
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3210..3229
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3318..3917)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3401..3421)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3898..3917
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3935..4351)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3951..3967)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3983..4005)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    4552..4751
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             4816..5285
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        5316..5420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5421..5489,5490..6281)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5639..5658)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6441..7029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6930..6949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7183..7200
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcggccctc tagatgcatg ctcgagttag
      781 attacatctt ctgcaactgt ggtgtcaaaa atctgatagc aaatttgatt atctgttcca
      841 tttgtgacat tgtatgactt gatgaagcat tccaaccacg gatatgcatc aatttttatt
      901 cctggaggac aaaacatatc catgatcata tccacacttt tctgaaggtc atcatccatc
      961 agaacttctg atgctggaat ctggaagaat ttgtcagaat ccatgagata ggcttctagt
     1021 actcctgttc catcatcaag tgtaaaggtc ataacaaaca catattggag gggtacaata
     1081 cccagtgctt ctgccacaga agaaggaatc cacgatgttt tatcaaccag ggaatttaga
     1141 ttttgtatgg atctcaaact agaacactgt ttacatccat agtgatgtat tgttccttgt
     1201 ataagaaatg gtgctgaaag gtccaaaagt tccaggtctt cgtggccaga tctcacagga
     1261 attacactat taaacttgtt cgagagtttg caaatttcac tgagtgtacc tccttctatc
     1321 aaaagtagac attcatttga aagcgggaga ataccattat ttttcacaaa atgaactgct
     1381 acttttcgtc ctttttgatt tttagtggtc cagatttttg aatcatataa tgatgtattt
     1441 tgtagcttga catctggggt tttagttgca ccatcctgaa aaattatatc caaatcgccc
     1501 tcatgtggaa cttcttgcag caaatgacat ttagggcaat gaagtttaac agactgaaat
     1561 agtcttctgg gcttatatga cctcaatttt gctcggatgc ggtattgttg aggagctttt
     1621 tgtttcaaaa tggcacatag tggtgtcctc tccaaatact gatgatctgt aagtattgta
     1681 gcagatagct gttgacatct ttctacctcg tataatgata ctgatccaga gcttggaaag
     1741 ctgtcgtcag gttctgattg acagataaca tctgaatgct gattggctgt caaatttgca
     1801 gattctaaat cctttttcag ttgatccaca tcagagttac tttctggcaa gaccctgatt
     1861 ccccgaccgt aactggtacc tccatgaaga tgaaactcta aacttaacat tgtctgattc
     1921 tctgaattca ttgattgaag tttggtatga aggctataga ttctaagaaa gcttccaacc
     1981 ttcagagatc ttgccacatg aacatggtta tcgtagacta aaatgtctat tgtcagattt
     2041 tgtagccgat ggatgtgact taaatcacct tcaagaacaa ggtcttgtat taagactctc
     2101 caagatggaa atggtgtcct ggtgccatcc cataccttta gaagaaatga tgctccgtcc
     2161 acttctgctt tgcccaagag ctgacaagtc aggtcaaaat actgcattgg ctgaacatca
     2221 cacaatttta gtaatgtcca agacggtgac atatgagtag atgcccaaac acgtaaggct
     2281 tctaccattt tgtggtcctc agtggatcct ctctttctta acaaatcttc ttcagaaatc
     2341 aacttttgtt ccatggtggc tgatccgagc tcggtaccaa gcttgggtct ccctatagtg
     2401 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
     2461 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2521 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     2581 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2641 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     2701 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     2761 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     2821 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     2881 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     2941 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     3001 aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac
     3061 atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa
     3121 gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca
     3181 agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     3241 ccatagggga tcgggagatc cgatgcatgg ggtcgtgcgc tcctttcggt cgggcgctgc
     3301 gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
     3361 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
     3421 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
     3481 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
     3541 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
     3601 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
     3661 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
     3721 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
     3781 acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac
     3841 gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg
     3901 ggcttgtact cggtcatggt aagctgatcc ggccggcgcc tagagaagga gtgagggctg
     3961 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
     4021 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
     4081 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
     4141 caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc
     4201 gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct
     4261 tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg
     4321 tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag
     4381 tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac gcgcggcttc
     4441 ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     4501 ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt cctcaggtcg
     4561 ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg ccgggtgttc
     4621 agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc
     4681 gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg
     4741 ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc
     4801 ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
     4861 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
     4921 gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg gttgtgggct
     4981 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
     5041 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
     5101 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
     5161 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     5221 tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt ttggcccata
     5281 ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5341 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5401 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5461 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5521 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5581 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5641 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5701 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5761 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5821 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5881 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5941 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6001 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6061 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6121 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6181 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6241 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6301 catatatact ttagattgat ttgcggccgc aaaaggatct aggtgaagat cctttttgat
     6361 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6421 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6481 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6541 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6601 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6661 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6721 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6781 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6841 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6901 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6961 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7021 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7081 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7141 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7201 gaagcggaag
//
